| .从鲐鱼消化道中提取蛋白水解酶的实验条件比较[J].中国海洋药物,1994,13(3):25-27. |
| 从鲐鱼消化道中提取蛋白水解酶的实验条件比较 |
| THE COMPARATION OF EXPERIMENTAL CONDITIONS EXTRACTING THE PROTEOLYTIC ENZYME FROM THE ALIMENTARY CANAL OF PNEUMATOPHORUS JAPONICUS |
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| DOI： |
| 中文关键词: 鲐鱼 消化道 蛋白水解酶 |
| English Keywords:Penmatophorus japonicus,alimentay canal,proteolytic enzyme, |
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| 中文摘要: |
| 本研究对从鲐鱼消化道中提取蛋白水解酶的几种实验条件进行了比较。结果表明,在用Cacl_2作活化剂的情况下,最适条件为:pH为7.5,抽提温度为20℃,提取时间2h,活化时间16h,乙醇沉淀浓度为70％。 |
| English Summary: |
| Various conditions were compared for extracting the proteolytic enxyme from the alimentary canal of Pneumatophorus juponicus (Houttuyn). The results showed that when using CaCl2 as activator, the suitable conditions were pH7. 5,extracting temperature 20 C,extracting time 2h,activiting time 16h,70% alcohol precipitaion. |
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