| .海洋酸性多糖J201A抑制肺成纤维细胞增殖活性的探讨[J].中国海洋药物,2002,21(3):1-4. |
| 海洋酸性多糖J201A抑制肺成纤维细胞增殖活性的探讨 |
| Inhibitory effect of laminarin (acidic polysaccharide) J20 1A on the proliferation of Human Lung Fibroblasts and its related mechanism of action |
| 修订日期：2001-11-20 |
| DOI： |
| 中文关键词: 海带酸性聚糖J201A 人胚肺成纤维细胞 细胞周期 蛋白质合成 受体 肺纤维化 |
| English Keywords:laminarin (acidic polysaccharide) J201A,human lung fibroblasts (HLF),cell cy-cle,protein synthesis,receptors |
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| 中文摘要: |
| 目的：研究海带酸性聚糖J201A对人胚肺成纤维细胞（HLF）体外增殖的抑制作用及其作用机制，方法：MTT法观察J201A对HLF体外增殖的抑制情况，流式细胞仪检测J201A对HLF增殖周期及蛋白质合成的影响；荧光染色法验证HLF上J201A受体的存在，结果：J201A体外能明显抑制HLF的增殖，将其阻抑在G0／G1期，能明显抑制其蛋白质的合成，且HLF上存在J201A的受体，结论：J201A可通过周期抑制和蛋白质合成抑制而阻止HLF的增殖，从而起到抗纤维化作用。 |
| English Summary: |
| To study the inhibitory effects of laminarin (acidic polysaccharide) J201A on the proliferation of human lung fibroblasts (HLF) and its related mechanism of action. Methods: Effect of J201A on the proliferation of HLF was evaluated by MTT assay, and the effects on cell cycle and synthesis of proteins of HLF were assessed by flow cytometry. The existence of J201A receptors on HLF was confirmed by fluorescent staining assay. Results:J201 A inhibited the synthesis of proteins and the proliferation of HLF at G0/G1 stage of cell cycle and J201A receptors existed on HLF. Conclusion:J201A exerted its antifibrotic activity by inhibiting HLF at G0/G1 stage of cell cycle and the synthesis of proteins. |
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