| .扇贝多肽对抗UVB辐照所致小鼠胸腺淋巴细胞凋亡的研究[J].中国海洋药物,2006,25(2):37-40. |
| 扇贝多肽对抗UVB辐照所致小鼠胸腺淋巴细胞凋亡的研究 |
| Study on the protective effect of polypeptide from Chlamys farreri (PCF) against murine thymocytes apoptosis induced by UVB radiation |
| 投稿时间：2005-03-01 |
| DOI： |
| 中文关键词: 扇贝多肽,中波紫外线,胸腺淋巴细胞,凋亡 |
| English Keywords:polypeptide from Chlamys farreri(PCF),UVB,thymocytes,apoptosis |
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| 中文摘要: |
| 目的研究扇贝多肽(PCF)对中波紫外线(UVB)辐照损伤小鼠胸腺淋巴细胞的保护作用。方法以UVB辐照制备损伤模型,预先给予PCF,应用透视电镜观察细胞超微结构的变化;免疫细胞化学检测细胞p53,Bcl-2,Bax基因蛋白的表达;原位杂交检测细胞P38mRNA的基因表达。结果UVB辐照引起小鼠胸腺淋巴细胞凋亡,PCF能明显减轻UVB辐射损伤引起的细胞超微结构改变。PCF还能抑制突变型p53蛋白和促凋亡蛋白Bax的表达,增强凋亡抑制蛋白Bcl-2的表达以及抑制P38 mRNA基因的表达。结论PCF对UVB辐照的体外小鼠胸腺淋巴细胞具有明显的保护作用。 |
| English Summary: |
| Objective To study the protective effect of PCF against murine thymocytes apoptosis induced by UVB irradiation.Methods The irradiation damage model of thymocytes caused by UVB was established.Cellular ultrastructuralalterations were observed with TEM.The expressions of P53,Bax and Bcl-2 proteins were examined by immunocytochemical technique. The p38 mRNA gene expression was also determined with the technique of in situ hybridization.Results Apoptotic thymocytes were observed after UVB irradiation.The ultrastructural damages of the cells induced by UVB irradiation were reduced by the treatment of PCF.PCF also raised the expression of Bcl-2 gene and decreased the expression of P53,Bax.The mRNA expressions of p38 were decreased in PCF groups.Conclusion PCF showed significant protective effects on UVB-irradiated murine thymocytes in vitro. |
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