| .提取琼胶糖的树脂新方法[J].中国海洋药物,2006,25(3):28-32. |
| 提取琼胶糖的树脂新方法 |
| A new method for extracting agarose using basic resin |
| 投稿时间：2005-10-17 |
| DOI： |
| 中文关键词: 琼胶糖,强碱性树脂,凝胶强度,结晶紫电泳,DNA凝胶电泳 |
| English Keywords:agarose,basic resin,gel strength,gentian violet electrophoresis,DNA gel electrophoresis |
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| 摘要点击次数: 2250 |
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| 中文摘要: |
| 目的研究一种低成本制备琼胶糖的简便方法。方法以江蓠琼胶粉为原料,采用NH4 型阳离子交换树脂脱除1.5%琼胶溶液中的金属离子,再用OH-型强碱性阴离子交换树脂在恒温70℃下吸附沉降琼胶酯,提取出白色琼胶糖。结果测定的灰分和硫酸根含量均符合电泳琼胶糖的指标,凝胶强度达到560g/cm2,提取率达57%,测定了其结晶紫电泳和DNA的琼胶糖凝胶电泳。结论本方法提取的琼胶糖具有良好的电泳性能,适合用于生物化学和分子生物学的凝胶电泳研究。本法与EDTA-2Na法和DEAE纤维素法相比,是一种低成本制备琼胶糖的简便方法。 |
| English Summary: |
| Objective To study a new method for extracting agarose. Methods In 1.5% agar solution, adding the cation resin exchanged by NH~ _ 4 to take off metal ions, then using the OH~ - basic resin to absorb agaropectin. Results and Conclusion The extracting rate was 57%, gel strength reached 560 g/ cm~ 2 , the agarose has good electricity function by measuring the gentian violet electrophoresis and the DNA gel electrophoresis, so it is suitable for bioche- mistry and molecular biology electrophoresis. It is a kind of simple and low cost method for extracting agarose which better than the EDTA- 2Na method and DEAE cellulose method. |
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