| 何颖,沈先荣,莫琳芳,等.方格星虫多糖对低剂量中子辐射损伤大鼠的保护作用[J].中国海洋药物,2014,33(3):49-55. |
| 方格星虫多糖对低剂量中子辐射损伤大鼠的保护作用 |
| Protective effects of Sipunculus nudus polysaccharides on radiation injury induced by low-dose neutron in rats |
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| 中文关键词: 关键词: 方格星虫多糖 低剂量中子辐射 抗氧化作用 胆固醇7- 羟化酶 |
| English Keywords:Keywords: Sipunculus nudus polysaccharides low-dose neutron irradiation antioxidation cholesterol 7-α-hydroxylase |
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| 中文摘要: |
| 摘要：目的 研究方格星虫多糖（SNPS）对低剂量中子辐射损伤大鼠的保护作用。方法 SNPS 3个剂量组70，140和280 mg kg-1。补充何种动物、数量、性别、级别，重量，SD大鼠，50只，雄性，清洁级，180-200 g，每天于照射前经口给予受试药，连续38 d。除正常对照组外，其余各组每天照射22 h，放射源为252Cf中子源，吸收剂量率为0.085 mGy h-1，累积吸收剂量为71.06 mGy。在照射第7、14、21、28、35 d检测外周血细胞计数，在照射38 d检测血清SOD和GSH-PX活力、MDA含量，血清胆固醇和甘油三酯含量，肝脏脂代谢相关基因CYP7A1、FABP1、PPARα mRNA表达变化，计算主要脏器指数。结果 与模型对照组比较，在照射第35 d，SNPS中剂量组外周血血小板显著回升，在照射第38 d，SNPS各剂量组血清GSH-PX活力显著上升，血清MDA含量显著下降，肝脏中胆固醇7- 羟化酶含量显著回升。结论 SNPS对长期低剂量中子辐射损伤大鼠具有升高血小板，提高抗氧化能力，调节胆固醇代谢等保护作用。 |
| English Summary: |
| Abstract: Objective To study the protective effects of Sipunculus nudus polysaccharides on radiation injury induced by low-dose neutron in rats. Methods Totally 50 male SD rats (sanitary degree，180-200 g) were administrated by intragastric infusion with SNPS at doses of 70, 140, 280 mg kg-1 before irradiation once a day for 38 days. Except for rats in the normal group, those in the rest groups were irradiated 22 h per day by 252Cf, the absorbed dose rate for 0.085 mGy h-1, integral absorbed dose for 71.06 mGy. At 7, 14, 21, 28, 35 d exposed irradiation, peripheral blood cells were counted. At 38 d exposed irradiation , the contents of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), total cholesterol (TC) and triglyceride (TG) in serum were detected. The genes expressions of CYP7A1 , FABP1 and PPARα in liver were analyzed. The indexes of main organs were calculated. Results Compared with model control group, peripheral blood PLT increased significantly in SNPSM group at 35 d exposed irradiation , GSH-PX activity and CYP7A1 (cholesterol 7-α-hydroxylase) gene expression increased significantly in SNPS groups, while the content of MDA decreased in SNPS groups at 38 d exposed irradiation . Conclusion SNPS may take a protective effect on rats injured by low-dose neutron irradiation with increasing PLT in serum, improving antioxidant activity and regulating cholesterol metabolism. |
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