| 宋文娟,颜洁,梁振,等.西沙海绵Agelas aff .nemoechinata化学成分研究△[J].中国海洋药物,2014,33(4):29-62. |
| 西沙海绵Agelas aff .nemoechinata化学成分研究△ |
| Studies on Chemical Constituents of XishaSponge Agelas aff .nemoechinata |
| 投稿时间：2014-03-04 修订日期：2014-04-11 |
| DOI： |
| 中文关键词: 海绵 化学成分 结构鉴定 细胞毒活性 |
| English Keywords:Sponge Chemical constituents Structural identification Cytotoxic activity |
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| 中文摘要: |
| 目的 研究西沙海绵Agelas aff .nemoechinata的化学成分。方法 利用Sephadex LH-20凝胶色谱、硅胶色谱、ODS色谱、HPLC等手段对化学成分进行了分离纯化；通过理化性质,波谱分析方法结合文献对照,鉴定化合物的结构。结果 从南海海绵甲醇溶剂提取物中,分离鉴定了8个单体化合物：尿嘧啶(1)、胸腺嘧啶(2)、1-H-吡啶酮(3)、胸腺嘧啶-2’-脱氧核苷(4)、2-甲酰胺吡咯(5)、3-醛基吲哚(6)、6-羟基-4,4,7a-三甲基-5,6,7,7a-四氢苯并呋喃-2(2H)酮(7)、5-氨基戊酸(8)。结论 化合物1~8均为首次从该种海绵中分离得到。化合物7 在50 μg/mL-1浓度水平,对K562白血病细胞株、P388白血病细胞株和HeLa肿瘤细胞株的抑制率分别是38.29%、23.23%和95.57%。 |
| English Summary: |
| Objective To study the constituents of Agelas aff .nemoechinata .Methods The isolation and purification of the compounds were performed by Sephadex LH-20, silica gel, ODS chromatography and HPLC methods. And their structures were determined by comparison their physicochemical characters and spectral data with literatures. Results From the methanol extracts , eight compounds were isolated and identified as Uracil (1)、Thymine (2)、1-H-pyridone (3)、2’-deoxythymidine (4)、2-pyrrole-carboxamide (5)、1H-indole-3-carbaldehyde (6)、6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (7) and 5-aminopentanoic acid (8). Conclusion Compounds 1-8 were isolated from this sponge for the first time. Compound 7 exhibited moderate cytotoxicity against K562, P388 and HeLa tumor cell lines with the inhibition proliferation 38.29%, 23.23% and 95.57%, at the concentration level of 50 μg/mL-1, respectively. |
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