| 胡敬峰,王远红,李帅,等.仿刺参糖胺聚糖对小鼠腹腔巨噬细胞功能的影响[J].中国海洋药物,2014,33(6):21-26. |
| 仿刺参糖胺聚糖对小鼠腹腔巨噬细胞功能的影响 |
| Effect of Apostichopus japonicus glycosaminoglycan on the function of peritoneal macrophages in mice |
| 投稿时间：2014-05-21 修订日期：2014-05-26 |
| DOI： |
| 中文关键词: 仿刺参糖胺聚糖 腹腔巨噬细胞 小鼠 免疫调节 |
| English Keywords:Apostichopus japonicus glycosaminoglycan peritoneal macrophages mice immunomodulatory |
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| 中文摘要: |
| 目的 研究仿刺参糖胺聚糖（HGAG）对小鼠腹腔巨噬细胞（Mφ）功能的影响，探讨其对小鼠的免疫调节作用。方法 不同浓度HGAG作用于体外培养Mφ，MTT比色法测定代谢活力；中性红法测定吞噬功能；试剂盒测定Mφ中乳酸脱氢酶（LDH）活性；Griess法测定NO生成量；ELISA法测定培养上清中TNF-α、IL-1β的分泌水平。结果 HGAG在0.1~25 μg·mL?1范围内对Mφ的功能有一定的促进作用。在1~10 μg·mL?1范围内与空白对照组相比可显著增强Mφ代谢活力和吞噬能力（P<0.01），提高LDH活力和NO生成量（P<0.05），显著增加培养上清中TNF-α和IL-1β（P<0.01）的浓度。与阳性对照组相比，在1~10 μg·mL?1范围内对Mφ吞噬功能以及TNF-α、IL-1β的生成有更好的促进作用（P<0.05），在高浓度（100μ g·mL?1）Mφ的各项功能指标均低于空白对照组。结论 HGAG在1~10 μg·mL?1浓度范围内可显著促进Mφ活性，并在一定程度上优于阳性对照组。表明HGAG能够激活Mφ，促进Mφ的功能，增强机体免疫。 |
| English Summary: |
| Objective To investigate the effects of Apostichopus japonicu glycosaminoglycan (HGAG) on the function of mice peritoneal macrophages (Mφ), and then explore the immunomodulatory effects in mice. Methods Different concentrations of HGAG were added to Mφ in vitro. Metabolic activity of Mφ was measured by MTT assay, the phagocytosis of neutral red was detected by colorimetric assay, lactate dehydrogenase (LDH) activity of macrophages was analyzed, nitric oxide (NO) was detected by Griess reagent, concentrations of TNF-α and IL-1β in culture supernatants were determined by ELISA. Results HGAG have a certain role in promoting the activity of Mφ within the range of 0.1~25 μg·mL?1. HGAG could enhance the metabolism and phagocytosis (P<0.01), increase LDH activity and NO production (P<0.05), improve TNF-α and IL-1β production (P<0.01) within a range of 1~10 μg·mL?1 compared with the control group. The phagocytosis, TNF-α and IL-1β production were increased significantly within a range of 1~10 μg·mL?1 compared with the positive control group (P<0.05). The Mφ function parameters of high concentration (100 μg·mL?1) group were lower than the control group. Conclusion Within the range of 1~10 μg·mL?1, HGAG can promote the activity of Mφ, and better than the positive control group to a certain extent. Indicated that HGAG can activate Mφ, promote the function of Mφ, strengthen the body immunity. |
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