| 王怀玲#,董栋#,金富军,等.琼枝麒麟菜多糖超声提取工艺及皮肤修复活性研究[J].中国海洋药物,2014,33(6):65-70. |
| 琼枝麒麟菜多糖超声提取工艺及皮肤修复活性研究 |
| #$NBSUltrasonic extraction#$NBSprocess optimization of polysaccharides from the Eucheuma gelatinae and#$NBStheir skin#$NBSrepair and protection activity#$NBSevaluation |
| 投稿时间：2014-08-11 修订日期：2014-09-10 |
| DOI： |
| 中文关键词: 琼枝麒麟菜 琼枝麒麟菜多糖 超声提取工艺 皮肤修复 |
| English Keywords:Eucheuma gelatinae Eucheuma gelatinae polysaccharose (EGP) ultrasonic extraction skin repair |
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| 中文摘要: |
| 目的 优化琼枝麒麟菜多糖(EGP)的超声提取方法，并探讨EGP在皮肤损伤修复方面的作用。方法 采用单因素考察和正交试验方法优化超声方法提取琼枝麒麟菜多糖(EGP)，苯酚-浓硫酸法测定EGP中多糖含量。通过MTT细胞毒性、细胞增殖实验和划痕实验，研究EGP对人永生化表皮细胞(HaCaT)形态、增殖分化和细胞迁移速度的影响，评价其在皮肤损伤修复方面的作用。结果 确定EGP的最优超声提取条件为：温度60 ℃，料液比1:100，时间30 min，EGP得率为40.3% ± 1.8%，其中多糖含量为48.2% ± 4.6%。EGP对HaCaT细胞几乎无毒，且在一定浓度范围内对HaCaT细胞的增殖有显著的促进作用；EGP还能加快HaCaT细胞的迁移速度，对皮肤损伤的愈合有显著的影响意义。结论 超声法提取EGP时间短，工艺稳定，所得EGP毒性小，对皮肤及粘膜损伤的修复具有促进作用，可广泛应用于化妆品及医疗器械。 |
| English Summary: |
| Objective We aimed to optimize the ultrasonic extraction process of Eucheuma gelatinae polysaccharides (EGP) and evaluate the EGP’s repair and protection activity. Methods The ultrasonic extraction process of EGP was optimized by single factor study and orthogonal experiment design, and the content of polysaccharide was measured using phenol-sulfuric acid method. We also evaluated the EGP’s repair and protection activity in terms of cell morphology, cell growth and cell migration on HaCaT cell line. Results It showed that the optimized extraction process was at the following condition: 60℃, solid-liquid ratio of 1:100, and 30 min, and EGP yielded 40.3% ± 1.8% which contented 48.2% ± 4.6% of polysaccharide. Cell proliferation and migration assays demonstrated that EGP played an important role to facilitate the HaCaT cell proliferation and migration with no toxicity which might accelerate skin repair and wound healing. Conclusion It was an energy saving and stable technology to extracted EGP by ultrasound. The extractive could improve the wound healing and regeneration of skin and mucosa membrane with no toxicity. EGP showed great potential in the fields of cosmetics and therapeutic devices. |
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