| 贺小玲,洪碧红.高效液相色谱法测定氨基葡萄糖拟肽的含量[J].中国海洋药物,2015,34(5):77-80. |
| 高效液相色谱法测定氨基葡萄糖拟肽的含量 |
| Determination of Glucosamine peptide mimetics by HPLC |
| 投稿时间：2015-01-22 修订日期：2015-02-09 |
| DOI： |
| 中文关键词: 高效液相色谱法 氨基葡萄糖拟肽 含量 |
| English Keywords:HPLC Glucosamine peptide mimetic Content |
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| 中文摘要: |
| 目的 采用Waters 2695型高效液相色谱仪建立定量分析氨基葡萄糖拟肽的方法。方法 以HypersilODS2 (4.6mm×250mm,5μm)为分离柱,流动相为5mmoL/L pH=7.5的磷酸二氢钾-乙腈体系 |
| English Summary: |
| Objective To establish a method to quantitatively analyze glucosamine peptide mimetics with HPLC. Methods Samples were separated on a Hypersil ODS2 column(5μm, 4.6mm × 250mm),the mobile phase consisted of 5 mmoL potassium dihydrogen phosphate- acetonitrile (pH =7.5,V/V) . The flow rate was 1. 0 mL/min . Results The standard curve of four samples were respectively linear over the range in 25 ~ 800.00μg/mL ( r = 0. 9999,TAG-Phe), 40 ~ 1000μg /mL(r = 0. 9993, TAG-Leu), 27 ~ 600.00μg /mL(r = 0. 9991, TAG-Glu)and 50 ~ 600.00μg /mL(r = 0. 9991, TAG-Val). The limit of quantitation were 20.00 ,35.54, 25.00 and 49.00μg / mL in turn (S/N = 10) .And limit of detection were 4.50, 15.30 , 13.45 and 19.30μg /mL (S/N = 3).The average recovery rate of four samples were 99%~101.42%%,day and inter-day precision were less than 1.37%.Conclusion The method is simple, rapid, specific, and suitable for the determination of glucosamine peptide mimetic. |
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