| 张晓磊,郝建华,郑鸿飞,等.1株产α-环糊精葡萄糖基转移酶菌株的产酶条件优化[J].中国海洋药物,2015,34(5):63-70. |
| 1株产α-环糊精葡萄糖基转移酶菌株的产酶条件优化 |
| Optimized fermentation of a cyclomaltodextrin glucanotransferase producing strain |
| 投稿时间：2015-03-25 修订日期：2015-04-29 |
| DOI： |
| 中文关键词: 海洋芽孢杆菌 α-环糊精葡萄糖基转移酶 产酶条件优化 响应面 |
| English Keywords:Bacillus, α-CGTase, optimized fermentation conditions, the response surface |
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| 中文摘要: |
| 目的提高菌株Y112产α-环糊精葡萄糖基转移酶（α-CGTase）的能力。方法采用单因素法和响应面法，对菌株Y112产酶的培养基成分和培养条件进行了优化。结果最终确定了菌株Y112产酶最佳培养基成分为：玉米淀粉 7.5 g/L，蛋白胨17.8 g/L，酵母浸粉 9.1 g/L，Mn2+ 0.6 mmol/L，NaCl 50 g/L，Na2CO3 15 g/L(pH 9.6)；最佳产酶的培养条件为：27 ℃下发酵50 h。结论优化后的酶活为优化前的2.8倍。 |
| English Summary: |
| Objective To increase the yield of cyclomaltodextrin glucanotransferase (CGTase) produced by strain Y112. Methods The fermentation conditions and medium of strain Y112 were studied by using single factor experiment and the response surface design. Results The optimized liquid medium for the production of α-CGTase was composed of corn starch 7.5 g/L, peptone 17.8 g/L, yeast extract 9.1 g/L, Mn2 0.6 mmol/L, NaCl 50 g/L, Na2CO3 15 g/L(pH 9.6). Incubation at 27 oC for 50 h resulted a high level of α-CGTase production. Conclusion The α-CGTase production was 2.8 times as high as the original conditions. |
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