| 赵芹,林栋,刘海梅.2种海参胶原蛋白多肽对血管内皮细胞保护作用的比较研究[J].中国海洋药物,2016,35(5):50-56. |
| 2种海参胶原蛋白多肽对血管内皮细胞保护作用的比较研究 |
| Comparative study on the protective effects of collagen polypeptides from two species of sea cucumber on vascular endothelial cells |
| 投稿时间：2016-01-22 修订日期：2016-03-16 |
| DOI： |
| 中文关键词: 海参 胶原蛋白多肽 动脉粥样硬化 抗氧化 血管内皮细胞 活性氨基酸 |
| English Keywords:sea cucumber collagen polypeptides atherosclerosis antioxidation vascular endothelial cell active amino acid |
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| 中文摘要: |
| 目的 观察革皮氏海参和北极刺参胶原蛋白多肽对氧化型低密度脂蛋白(ox-LDL)损伤的血管内皮细胞的保护作用，探讨其保护内皮细胞的作用机制。方法 采用ox-LDL处理血管内皮细胞(ECV304)建立氧化应激损伤模型，以MTT法测定ECV304的增殖活性，硫代巴比妥酸法测定细胞内的丙二醛(MDA)含量，比色法测定一氧化氮合酶(NOS)活力和一氧化氮(NO)释放量，Hoechst33258染色法检测细胞的凋亡，Western blotting法检测caspase-3蛋白的表达。结果 经2种海参胶原蛋白多肽预处理后，ECV304细胞的增殖率显著升高 (P<0.05，P<0.01)，细胞凋亡比率和MDA含量显著降低(P<0.05，P<0.01)，细胞NOS活力和NO释放量均显著提高 (P<0.05，P<0.01)，凋亡蛋白caspase-3的表达量显著降低。结论 2种海参胶原蛋白多肽均能有效保护脂质过氧化物损伤的血管内皮细胞，其中北极刺参胶原蛋白多肽在抑制细胞凋亡和提高NOS活性方面效果更突出，可能与其氨基酸组成有关。 |
| English Summary: |
| Objective To investigate the protective effects of collagen polypeptides from Peasonothria.graeffei and Thelenata. anax on vascular endothelial cells damaged by oxidized low density lipoprotein (ox-LDL). Methods Human umbilical vein endothelial cells (ECV304) had been cultured in vitro to establish a model of endothelial cell oxidative damage induced by ox-LDL. MTT assay was used to test the proliferation activity of endothelial cell. The content of MDA was examined by thiobarbituric acid assay. The activity of NOS and the content of NO were determined by colorimetric method. Apoptosis of vascular endothelial cells was measured by Hoechst 33258 staining. Expression of apoptosis-related protein caspase-3 was examined by western blotting. Results All doses of collagen polypeptide could promote the growth of ECV304 (P<0.05, P<0.01) and inhibit cell apoptosis induced by ox-LDL observably (P<0.05, P<0.01). The content of MDA was decreased significantly (P<0.05, P<0.01), NOS activity and NO content were stimulated remarkably (P<0.05, P<0.01). The expression of caspase-3 protein was significantly decreased. Conclusion Collagen polypeptides from two species of sea cucumber could play a protective role in vascular endothelial cells oxidative damage induced by ox-LDL. The significant effect on cell apoptosis inhibition and NOS activity regulation of T. Anax correlated to its active amino acids composition in all probability. |
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