| 王姝垚,刘志纯,秦玲,等.海洋硫酸多糖PAE的结构和抗病毒活性研究[J].中国海洋药物,2019,38(1):17-22. |
| 海洋硫酸多糖PAE的结构和抗病毒活性研究 |
| Structural characterization and antiviral activityof the marine sulfated polysaccharide PAE |
| 投稿时间：2018-10-31 修订日期：2018-12-25 |
| DOI： |
| 中文关键词: 硫酸多糖 结构表征 抗病毒活性 |
| English Keywords:sulfated polysaccharide structural characterization antiviral activity |
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| 中文摘要: |
| 目的 对肠浒苔来源的硫酸多糖的结构和抗病毒活性进行研究。 方法 通过稀碱提取法,从肠浒苔中提取硫酸多糖,采用强阴离子交换色谱和凝胶渗透色谱对多糖进行分离纯化；采用高效液相色谱(HPLC)、高效凝胶渗透色谱(HPGPC)、傅里叶变换红外光谱(IR)以及气质联用色谱(GC-MS)方法对多糖的结构进行表征；采用细胞病变效应测定PAE对不同病毒的抑制率。 结果 从肠浒苔中分离得到多糖PAE,其分子量为13.98 kDa,主要由鼠李糖、葡萄糖醛酸和木糖构成,糖链主要由(1→4)-Rhap,(1→3,4)-Rhap,(1→2,4)-Rhap,Xylp(1→和(1→4)-Glcp组成,硫酸根主要位于(1→4)-Rhap 的C-2或C-3位以及Xylp(1→的C-4位上,PAE具有良好的抗病毒活性,特别是对呼吸道合胞体病毒的抑制率较高。 结论 海洋多糖PAE是一种结构新颖的由鼠李糖、葡萄糖醛酸和木糖组成的硫酸多糖,具有良好的抗病毒活性。 |
| English Summary: |
| Objective To investigate the structural characteristics and antiviral activity of a sulfated polysaccharide from Entermorpha intestinalis. Methods Sulfated polysaccharide was obtained by extraction with alkali solution, and purified by Q-Sepharose Fast Flow anion-exchange and Sephacryl S-400 gel-permeation chromatography. The structural characteristics of the polysaccharide were identified by high performance liquid chromatography (HPLC), high gel permeation chromatography (HPGPC), Fourier-transform infrared (IR) and gas chromatography-mass spectrometry (GC-MS). The antiviral activity in vitro of PAE was assessed by cytopathic effect inhibition. Results The polysaccharide PAE was obtained from Entermorpha intestinalis, and its average molecular weight was estimated to be about 13.98 kDa. PAE was mainly composed of rhamnose, glucouronic acid and xylose. The chain of PAE primarily consisted of (1→4)-Rhap,(1→3,4)-Rhap,(1→2,4)-Rhap,Xylp(1→ and (1→4)-Glcp. The position of sulfate ester was at C-2 or C-3 position of (1→4)-Rhap and C-4 position of Xylp(1→. PAE was non-cytotoxic and obviously inhibited various viruses, especially for the human respiratory syncytial virus. Conclusion The polysaccharide PAE was a novel sulfated xylglucuronorhamnan with significant antiviral activity. |
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