| 张惠姝,张添,张林铃,等.海洋双吲哚化合物溶解大鼠脑微血栓的纤溶作用[J].中国海洋药物,2023,42(6):41-47. |
| 海洋双吲哚化合物溶解大鼠脑微血栓的纤溶作用 |
| Fibrinolytic Effect of Marine Bisindole Compounds in Dissolving Cerebral Microthrombosis of Rats |
| 投稿时间：2022-05-26 修订日期：2022-09-13 |
| DOI：10.13400/j.cnki.cjmd.2023.06.022 |
| 中文关键词: 脑微血栓 FGFC1 血栓成像 组织切片 纤维蛋白降解产物 |
| English Keywords:Cerebral microthrombosis FGFC1 Low-field MRI thrombography Tissue section Fibrin degradation products |
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| 中文摘要: |
| 目的 检测海洋双吲哚生物碱FGFC1对大鼠脑微血栓的纤溶作用。方法 静脉注射异硫氰酸荧光素-纤维蛋白诱导大鼠脑微血栓形成，低场核磁共振血栓成像和组织切片评价脑微血栓动物模型构建；颈静脉注射给药FGFC1，荧光比色法检测大鼠血浆荧光强度，ELISA方法检测纤维蛋白降解产物和纤溶酶原活性。结果 低场核磁共振血栓成像和HE染色组织切片显示异硫氰酸荧光素-纤维蛋白诱导大鼠脑部密度增强和脑血管管壁增厚；冷冻组织切片显示脑血管存在荧光亮斑；血浆荧光强度显示2 h时异硫氰酸荧光素-纤维蛋白诱导大鼠脑微血栓形成；当FGFC1给药剂量为5 mg/kg时，给药后2 h大鼠血浆FDP、D-D及PLG活性分别为595.24 ng/mL、5.70 ng/mL和86.98 IU/L，与空白对照组差异显著。结论 异硫氰酸荧光素-纤维蛋白诱导大鼠形成脑微血栓模型，FGFC1能够增强脑微血栓动物模型的纤溶活性，实现脑微血栓溶解；海洋纤溶化合物FGFC1具有发展为溶解脑微血栓药物的潜力。 |
| English Summary: |
| Objective To detect the fibrinolytic effects of the marine bisindole alkaloids FGFC1 on cerebral microthrombosis of rats. Methods Fluorescein isothiocyanate-fibrin (FITC-fibrin) was injected intravenously to induce cerebral microthrombosis in rats, and the animal models of cerebral microthrombosis were constructed by low-field MRI thrombus images and tissue sections; FGFC1 was injected intravenously, fluorescence intensity of plasma was detected by fluorescence colorimetry, fibrin degradation products and fibrinogen activity were detected by ELISA. Results Low-field MRI thrombus images and HE staining tissue sections showed fluorescein isothiocyanate-fibrin induced brain density enhanced and cerebral vascular wall thickened in rats; frozen tissue sections showed fluorescent bright spots in cerebral vessels; fluorescence intensity of plasma showed FITC-fibrin induced cerebral microthrombosis in rats at 2 h; when dose of FGFC1 was 5 mg/kg, FDP concentrations, D-D concentrations and PLG activities of plasma in rats were 595.24 ng/mL, 5.70 ng/mL and 86.98 IU/L, respectively, at 2 h after administration, which were significantly different from the cerebral microthrombosis control group. Conclusion FITC-fibrin induced the formation of cerebral microthrombosis in rats, FGFC1 was enhanced the fibrinolytic activity and cerebral microthrombosis was dissolved. It was demonstrated that the marine fibrinolytic compound FGFC1 has the potential to be developed as a drug for cerebral microthrombosis. |
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