汲蕾,李兆锟,王鹏越,等.毛蚶共附生Aspergillus clavatonanicus JL001含氮类次级代谢产物的研究[J].中国海洋药物,2023,42(5):65-70.
毛蚶共附生Aspergillus clavatonanicus JL001含氮类次级代谢产物的研究
Study on the nitrogen containing secondary metabolites from symbiotic Aspergillus clavatonanicus JL001 isolated from Scapharca subcrenata
投稿时间：2022-06-08  修订日期：2022-08-29
DOI：10.13400/j.cnki.cjmd.2023.05.002
中文关键词:  毛蚶  共附生Aspergillus clavatonanicus JL001真菌  细胞毒活性  含氮类化合物
English Keywords:Scapharca subcrenata  symbiotic fungus Aspergillus clavatonanicus JL001  cytotoxic activity  nitrogen containing secondary metabolites
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作者单位E-mail
汲蕾 滨州医学院药学院 1369267210@qq.com 
李兆锟 滨州医学院药学院 646585974@qq.com 
王鹏越 滨州医学院 2511594589@qq.com 
李倩倩 滨州医学院药学院  
刘为忠 滨州医学院药学院  
刘德胜* 滨州医学院药学院 desheng_liu@sina.com 
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中文摘要:
      目的 研究毛蚶共附生Aspergillus clavatonanicus JL001的含氮类次级代谢产物，并探索其细胞毒活性。方法 静置发酵后，萃取得到粗提物。采用正反相硅胶色谱法、半制备高效液相、制备薄层色谱法（TLC ）分离纯化真菌的次级代谢产物，根据化合物NMR和HRMS数据结合文献报道确定其结构。利用CCK8法测定次级代谢产物1～5对人肿瘤细胞HepG2的细胞毒活性。结果 从毛蚶共附生Aspergillus clavatonanicus JL001中分离得到了8个含氮类化合物，分别为tryptoquivaline L（1）、quinadoline A（2）、scequinadoline I（3）、prelapatin B（4）、clavatustide B（5）、环（(苯丙-丙)二肽（6）、环（(酪-亮)）二肽（7）和环（(酪-异亮)）二肽（8）。其中化合物3和4对HepG2细胞具有一定的增殖抑制作用。结论 研究结果为从海洋动物共附生真菌中寻找新型抗生素提供参考和依据。
English Summary:
      Objective To study the nitrogen containing secondary metabolites from a symbiotic Aspergillus clavatonanicus JL001 isolated from Scapharca subcrenata and assessment of their cytotoxicity on HepG2 cell line. Methods After static fermentation, the crude extract was obtained and then subjected to normal or reverse silica column, semi-preparative HPLC, preparative TLC to get the secondary metabolites. The structures of the isolated compounds were determined by the interpretation of NMR and MS data, along with the comparison with the literature data. The cytotoxic activity of compounds 1～51～?5 towards HepG2 cell lines was performed by CCK8 method. Results Eight nitrogen containing compounds were purified and identified as tryptoquivaline L (1), quinadoline A (2), scequinadoline I (3), prelapatin B (4), clavatustide B (5), cyclo(Phe-Ala) (6), cyclo(Tyr-Leu) (7) and cyclo(Tyr-Ile) (8). Among them, compounds 3 and 4 showed anti-proliferative activity towards HepG2 cell lines. Conclusion The findings provide a research basis for development of new drug resources from symbiotic Aspergillus clavatonanicus.
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