| 钱亚,王有杰,郭铠溶,等.南海冷泉沉积物来源真菌Penicillium guanacastense HDN20-0005次级代谢产物研究[J].中国海洋药物,2024,43(4):12-18. |
| 南海冷泉沉积物来源真菌Penicillium guanacastense HDN20-0005次级代谢产物研究 |
| Secondary metabolites of South China Sea cold seep sediment-derived fungus Penicillium guanacastense HDN20-0005 |
| 投稿时间：2023-03-28 修订日期：2023-04-28 |
| DOI：10.13400/j.cnki.cjmd.2024.04.002 |
| 中文关键词: 中国南海 冷泉 真菌 次级代谢产物 |
| English Keywords:South China Sea cold seep fungus secondary metabolites |
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| 目的 研究南海冷泉来源真菌Penicillium guanacastense HDN20-0005的次级代谢产物。方法 优化菌株培养条件,运用硅胶柱层析、ODS反相柱层析和半制备高效液相色谱法(HPLC)对其发酵产物进行分离提纯,通过质谱(MS)、核磁共振波谱(NMR)、计算ECD、DP4+分析及和已报道文献比对,对化合物结构进行解析,并进行抑菌活性评价。结果 从该菌株中分离得到1个新的酸酐类化合物(R,Z)-3-ethyl-6-methyl-3,4-dihydro-2H-1,4-oxazocine-2,5,8-trione(1),1个新的色酮衍生物(E)-6,7-dihydroxy-3-(3-oxobut-1-en-1-yl)-4H-chromen-4-one(2),1个已知色酮衍生物penialidin F(3)和1个已知化合物pencolide(4)。评价了化合物1～4对7种真菌和细菌的抑制活性,结果表明化合物均无抑菌活性。此外，评价了新化合物1和2的黄嘌呤氧化酶抑制活性，结果显示化合物2具有微弱的黄嘌呤氧化酶抑制活性，抑制率为（13.16 ± 1.57）%。 |
| English Summary: |
| Objective To study the secondary metabolites of South China Sea cold seep sediment-derived fungus Penicillium guanacastense HDN20-0005. Methods The culture conditions of the strain were optimized, and the fermented products were isolated and purified by silica gel column chromatography, ODS reverse phase column chromatography, and semi-preparative high-performance liquid chromatography (HPLC). The structures of compounds were analyzed by mass spectrometry (MS), nuclear magnetic resonance spectroscopy (NMR), calculation of ECD, DP4+ analysis, and comparison with reported literature. The antimicrobial activity of the compounds was evaluated. Results One new anhydride compound, (R,Z)-3-ethyl-6-methyl-3,4-dihydro-2H-1,4-oxazocine-2,5,8-trione (1), one new chromone derivative, (E)-6,7-dihydroxy-3-(3-oxobut-1-en-1-yl)-4H-chromen-4-one (2), one known chromone derivative, Penialidin F (3), and one known compound, pencolide (4), were isolated from the strain. The antimicrobial activity of compounds 1-4 against seven fungi and bacteria was evaluated, and the results showed that all compounds had no antimicrobial activity. In addition, the inhibitory activity of xanthine oxidase was evaluated for the new compounds 1 and 2, and the results showed that compound 2 exhibited weak inhibitory activity against xanthine oxidase with an inhibition rate of (13.16 ± 1.57)%. |
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