| 于曦,徐新颖,刘英娟,等.低分子量褐藻胶古罗糖醛酸对铜负荷小鼠的治疗作用机制研究[J].中国海洋药物,2025,44(2):43-49. |
| 低分子量褐藻胶古罗糖醛酸对铜负荷小鼠的治疗作用机制研究 |
| The therapeutic effect and possible mechanism of low molecular weight algin guluronic acid in copper-loaded mice |
| 投稿时间：2023-10-09 修订日期：2023-10-26 |
| DOI：10.13400/j.cnki.cjmd.2025.02.008 |
| 中文关键词: 低分子量褐藻胶 古罗糖醛酸 铜负荷 小鼠 肝 |
| English Keywords:low molecular weight algin guluronic acid copper load mice liver |
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| 中文摘要: |
| 目的 探讨低分子量褐藻胶古罗糖醛酸(LMWA-PG)对铜负荷小鼠的治疗作用和可能的机制,为防治Wilson病提供新的思路。方法 健康雄性昆明小鼠50只,应用铜负荷饮食法建立铜负荷模型。治疗组小鼠给予不同浓度LMWA-PG灌胃治疗,对照组小鼠同步给予等体积的生理盐水灌胃,每日1次,连续60天。通过动物行为学评价小鼠的神经行为功能；生化法血清检测小鼠的肝功指标：血清总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB),计算白球比值(A/G)；Westen Blot检测小鼠肝细胞CER、p-AMPK的表达。结果 造模后小鼠体重与较对照组显著下降(P<0.05)；转棒时间显著缩短(P<0.05),爬杆时间显著延长(P<0.01)；总蛋白水平显著下降(P<0.05),白球比显著上升(P<0.05)；CRE表达水平显著增强(P<0.05),p-AMPK/AMPK水平显著升高(P<0.05)。给予不同浓度LMWA-PG治疗后,高剂量组小鼠的体重较模型组组显著增加(P<0.05)；转棒时间显著延长(P<0.01),爬杆时间显著缩短(P<0.05)；总蛋白水平显著升高(P<0.01),白球比值显著回落(P<0.05)；CER表达水平显著减弱,p-AMPK水平显著降低(P<0.01)。结论 LMWA-PG能够吸附和排泄铜离子,减轻炎症反应,改善铜负荷小鼠肝细胞代谢,从而对Wilson病有积极作用。 |
| English Summary: |
| Objective To explore the therapeutic effect and possible mechanism of low molecular weight align guluronic acid (LMWA-PG) on the copper-loaded mice, so as to provide a new idea for the prevention and treatment of Wilson"s disease. Methods Fifty healthy male Kunming mice were fed copper-loaded diet to establish the copper-loaded mouse model. The mice in treatment group were given different concentrations of LMWA-PG by gavage, while the mice in control group were orally given same amount of normal solution simutaneoisly 1 time per day for 60 days. The neurobehavioral function of mice was evaluated by animal behavior; the liver function indexes of mice such as serum levels of total protein (TP), albumin (ALB) , globulin (GLB) were detected by biochemical assay, and the ratio of ALB/GLB (A/G) was calculated; the expression levels of CER and p-AMPK in hepatic cells were detected by Western Blotting. Results Compared with control group, the weight of mice in model (Copper) group decreased significantly (P<0.05); The time of rotating rod shortened significantly (P<0.05) and the climbing time prolonged significantly (P<0.01); The serum level of TB decreased significantly (P<0.05), while the ratio of A/G was significantly increased (P<0.05); The expression of CRE enhanced significantly (P<0.05), and the level of p-AMPK/AMPK was significantly increased (P<0.05). After treated with different concentrations of LMWA-PG, the weight of mice in Copper+PG-H group was significantly higher than that in Copper group (P<0.05); The time of rotating rod prolonged significantly (P<0.01) and the climbing time shortened significantly (P<0.05); The serum level of TB increased significantly (P<0.01), while the ratio of A/G was significantly dropped (P < 0.05); The expression of CER reduced significantly (P<0.01) and the level of p-AMPK/AMPK was significantly decreased (P<0.01). Conclusion LMWA-PG could adsorb and excrete copper ions, reduce inflammation reaction, improve the hepatic metabolism of copper-loaded mice, so that to act a positive effect on Wilson"s disease. |
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