| .海洋嗜盐微生物的研究开发：Ⅱ.嗜盐细菌产生β—胡萝卜素[J].中国海洋药物,1996,15(4):1-4. |
| 海洋嗜盐微生物的研究开发：Ⅱ.嗜盐细菌产生β—胡萝卜素 |
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| DOI： |
| 中文关键词: 嗜盐细菌 β－胡萝卜素 类胡萝卜素 海洋药物 |
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| 中文摘要: |
| 从各种海泥分离纯化到多株海洋嗜盐细菌，经筛选发现其中Ｆ－５菌株等属于嗜盐杆菌属，能产生胡萝卜素，在经选定的最适条件下发酵时，其所产生的胡萝卜素经提取和纯化，获得紫红色的结晶，通过现代化波谱技术，包括紫外和红外吸收光谱，质谱，质子和碳－１３－磁共振谱的鉴定，确认是β－胡萝卜素，除Ｆ－５菌株外，还有一些待鉴定的菌株也能产生胡萝卜素。这是嗜盐细菌能产生β－胡萝卜素的首例报告。 |
| English Summary: |
| Strain F-5 and several other strains of Halobacteria were isolated from saltern pool of Xiamen, China. Strain F-5 is Gram-negative rod and obligate aerob'e. Optimum salt concentration for strain F-5 growth is 18% to 26% NaCl and no growth below 14% NaCl. And strain F-5 can produce carotenoids pigments. Therefore, strain F-5 is considered as one member of extreme Halogacteria.
Natural pigment F-5-C1 is mainly produced by strain F-5. F-5-C1 is obtained as a purple crystal and soluble in organic solvents, but no soluble in water. F5-C1 exhibits a molecular ion by EI-MS at m/z 536. 9, corresponding to the formula C40H56. The UV and IR spectra of F-5-C1 is identical with that of fj-carotene (E. Merck product), which was isolated from carrot. These informations coupled with 'HNMR, 13CNMR and MS spectral data allowed us to consider that F-5-C1 is identified with B-carotene. And this is the first report on B-carotene produced by one strain of Halobacteria. |
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