| .藻蓝蛋白对小鼠粒单系祖细胞生成的影响[J].中国海洋药物,1996,15(4):25-28. |
| 藻蓝蛋白对小鼠粒单系祖细胞生成的影响 |
| EFFECT OF C - PHYCOCYANIN ON GRANULOPOIESIS AND MONOCYTOPOIESIS IN MICE |
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| DOI： |
| 中文关键词: 钝顶螺旋藻 藻蓝蛋白 粒单系祖细胞 海洋药 |
| English Keywords:Spirulina platensis,c- phycocyanin,granulopoiesis and monocytopoiesis,colony stimulating activity (CSA) |
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| 中文摘要: |
| 藻蓝蛋白是从钝旋藻中分离，纯化所得。采用造血祖细胞体外培养技术，研究了Ｃ－ＰＣ对小鼠粒单系祖细胞生成的影响。实验结果表明，Ｃ－ＰＣ能够提高粒单系祖细胞生成，亦能明显提高正常小鼠血清的集落刺激活性。Ｃ－ＰＣ体内或体外刺激制备的小鼠脾细胞条件培养液能明显增加ＣＦＵ－ＧＭ的集落数。在有集落刺激因子存在时，体外加入Ｃ－ＰＣ培养粒单系祖细胞，Ｃ－ＰＣ能明显提高ＣＦＵ－ＧＭ的集落生成率。 |
| English Summary: |
| C - phycocyanin (C - PC) was isolated and purified from blue - green alga Spirulina platensis. By using the thechnique of culture of hemopoietic progenitor cell in vitro, we studied the effect of C -PC on granulopoiesis and monocytopoiesis in mice. The results indicate that C -PC can enhance the proliferation of CFU -GM, promote the granulopoiesis and monocytopoiesis in mice. The C -PC can markedly increase the colony stimulating activity of serum in normal mice. The prepared serum -free spleen cell conditioned media stimulated by C -PC in vivo and in vitro provided the only increase in CFU - GM colony formation. After in -vitro addition of C -PC in the presence of GM -CSF, there was a pronounced increase in the number of CFU -GM colonies. |
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