.紫球藻细胞中AA,EPA及色素提取的研究[J].中国海洋药物,1999,18(2):28-31. |
紫球藻细胞中AA,EPA及色素提取的研究 |
STUDIES ON LIPIDS IN DIFFERENT KIND CELLS OF POR- PHYRIDIUM CRUENTUM |
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DOI: |
中文关键词: 紫球藻 花生四烯酸 二十碳五烯酸 色素 |
English Keywords:Porphyridium cruentum,arachidonic acid,eicosapentaenoic acid,pigment |
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中文摘要: |
本文用多种有机溶剂对紫球藻中的类脂进行提取,利用气相色谱法对提取物中AA和EPA的含量进行了分析测定,并用光谱吸收法比较了提取脂怀物质-色素的含量。结果表明,氯仿-甲醇和乙醇是两种有效的提取剂,提取效果较好,紫球藻细胞中类脂含量为8-12%,并且因培养条件不同细胞中的类脂含量也有所不同。 |
English Summary: |
Several organic extractants were used to separate the lipids in a kind of red microalga-Porphyridium cruentum,and the content of AA and EPA in the liplds were analyzed by the means of gas chromatograghy. The results showed that chloroform-methanol and alcohol were the effective extractants, and the big cells had much more lipids than the small cells. |
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