| .红藻藻蓝蛋白对HL-60细胞生长的抑制作用[J].中国海洋药物,2000,19(1):. |
| 红藻藻蓝蛋白对HL-60细胞生长的抑制作用 |
| THE INHIBITION OF PHYCICYANIN FROM PORPHYRA HAITANENSIS ON THE GROWTH OF HUMAN LEUKEMIA HL-60 CELLS |
| |
| DOI： |
| 中文关键词: 红藻藻蓝蛋白,HL-60,半固体琼脂培养法,MTT法,流式细胞仪,免疫印渍法 |
| English Keywords:Phycocyanin from Porphyra Haitanensis, HL-60 cells Semi-solid agar, MTT staining assay, Flowcytometric assay, Westem Blot analysis. |
| Fund Project: |
|
|
| 摘要点击次数: 2316 |
| 全文下载次数: 27 |
| 中文摘要: |
| 本文运用半固体琼脂培养法及 MTT法研究了红藻藻蓝蛋白 (R- PC)对 HL- 60细胞生长的影响 ,结果发现 :R- PC能够显著抑制细胞的生长 ,且存在浓度效应和时间效应关系。为进一步探索 R- PC抑制 HL- 60细胞生长的可能机制 ,将不同浓度的 R- PC与 HL- 60细胞相互作用 6d后 ,分别用流式细胞仪检测 HL- 60细胞所处细胞周期中的时相和免疫印渍法测定 HL- 60细胞中 Bcl- 2基因表达产物的变化 ,实验结果显示 :R- PC确能使更多的 HL- 60细胞滞留于细胞周期的 G0 - G1 期 ,但与 R- PC浓度无正向关系 ,且 HL- 60细胞中 Bcl- 2基因表达产物的量也没有明显的变化。结果表明 :R- PC可能通过不同于 G0 - G1 期滞留及 Bcl- 2基因表达的机制抑制 HL- 60细胞的生长。 |
| English Summary: |
| The effect of phycocyanin from Porphyra haitanensis on the growth of human leukemia HL-60 cells was studied by semi-solid culture method and MTT staining assay. The results showed that the phycocyanin could significantly inhibit the growth of HL-60 cells in a dose- an time-dependent manner. In order to elucidate the potential pathway of the phycocyanin affecting the growth of HL-60 cells treated with phycocyanin at different concentrations for 6 days were further checked by flow cytometric assay and western blot analysis. Flow cytometric assay demonstrated that more HL-60 cells underwent G 0-G 1 arrest,but there were not evident differences between the experimental groups and the control. Western bolt analysis indicated that the expression level of Bcl-2 protein remained unchanged.These findings suggested that the phycocyanin could inhibit the growth of HL-60 cells by the potential pathways other than G 0-G 1 arrested or the expression change of Bcl-2 protein. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|