| .扇贝多肽对淋巴细胞的保护作用和反相液相色谱分析[J].中国海洋药物,2000,19(5):27-29. |
| 扇贝多肽对淋巴细胞的保护作用和反相液相色谱分析 |
| THE PROTECTIVE ACTION OF PCF ON LYMPHOCYTE AND THE ANALYSIS OF IT BY A REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RPLC) |
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| DOI： |
| 中文关键词: 淋巴细胞 免疫作用 反相液相色谱 扇贝多肽 |
| English Keywords:Polypeptide, Lymphocyte, Inhibition, Reversed phase high performance liquid chromatography (RPLC) |
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| 中文摘要: |
| 为进一步研究扇贝多肽对淋马细胞的作用及其成分。采用地塞米松与脾脏和胸腺淋巴细胞共同培养，建立了淋巴细胞免疫抑制实验模型。并利用反人相色谱法对ＰＣＦ进行了分析。结果表明，ＤＥＸ能显著降低脾脏和胸腺淋巴细胞的活性，ＤＥＸ组吸收度值明显降低；而ＰＣＦ不仅能显著地减轻其对免疫细胞的抑制作用，同时还可以促进免疫细胞的活性，使ＤＥＸ＋ＰＣＦ组吸收度值明显增高。相对分子质量为８００～１０００Ｄ的ＰＣＦ经ＲＰＬＣ |
| English Summary: |
| In order to study the inhibitory function of the PCF on lymphocyte and the composition of it, the culture of the lymphocyte of spleen and thymus with DEX was used as an experimental model of inhibitory immunity for the lymphocyte. PCF was assayed by RPLC. The results showed: DEX can reduce the activities of lymphocyte in spleen and thymus, and cause a significant reduction of A value for DEX group (P<0.01) and a significant rise of A value of DEX PCF group (P<0.01), after the analysis of the PCF (Mr=800 1000D) which has the four kind of retention time 3.923,4.865,5.033,5.441. These result indicate that PCF may be albe to protect the lymphocyte from the inhibition caused by DEX. |
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