| .乌贼墨诱导小鼠巨噬细胞产生白细胞介素-1的研究[J].中国海洋药物,2003,22(5):17-19. |
| 乌贼墨诱导小鼠巨噬细胞产生白细胞介素-1的研究 |
| Study on Secretion of Interleukin-1 Induced by Sepia in Mice |
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| DOI： |
| 中文关键词: 乌贼墨 IL-1 巨噬细胞 成纤维细胞 |
| English Keywords:Sepia,IL-1,macrophage,fibroblast, |
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| 中文摘要: |
| 目的：应用乌贼墨，研究其诱导BALB／C鼠巨噬细胞产生白细胞介素-1(interleukin-1)。方法：给小鼠ig灌入乌贼墨，抽取腹腔液，应用成纤维细胞增殖实验检测诱导巨噬细胞产生IL-1的生物学活性，体内持续时闰争不同稀释浓度促细胞增殖的结果。结果：乌贼墨能诱导BALB／C鼠巨噬细胞产生IL-1，且分泌活性较强，与对照组比较有显著性差异。体内跟踪检测可见停止诱生后第2天达高峰，第5天分泌活性仍明显，第9天接近正常。lL-1待检液稀释8倍时活性无明显变化，32倍时近正常水平。结论：乌贼墨能诱导小鼠巨噬细胞产生IL-1，48h分泌活性最高。 |
| English Summary: |
| Objective To investigate secretion of interleukin-1 (IL-1) in BALB/C mice macro-phage induced by sepia. Methods Ascitic fluid was drawed out after sepia was poured into mice intragastrically and the biologic activity of IL-1 induced in mice macrophage was examined by fibroblast proliferation experiment. Duration of IL-1 secretion and the concentration- activity relation in vivo were also studied. Results Sepia can induce IL-1 in BALB/C mice macrophage. The effects had significant differences compared with control group. Tracking detection in vivo displayed IL-1 had the highest secretion at the second day after stopped inducing and also secretion evidence at the fifth day and reached to normal at the ninth day. The IL-1 activity of original-detected liquid had not decreased at 8 fold dilution and approach normal at 32 fold. Conclusion Sepia can induce IL-1 in BALB/C mice macrophage, and it had shown the highest secretion was at 48 hours. |
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