| .高温低盐压力对缢蛏血淋巴碱性蛋白影响机理的研究[J].中国海洋药物,2004,23(3):23-27. |
| 高温低盐压力对缢蛏血淋巴碱性蛋白影响机理的研究 |
| Influence mechanisms of high temperature and low salinity on hemolymph basic proteins of Sinonovacula constricta |
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| 中文关键词: 双相电泳 图像分析 缢蛏 血淋巴碱性蛋白 高温低盐 |
| English Keywords:biphase electrophoresis,image analysis,Sinonovacula constricta (Lamarck),basic protein in hemolymph,high temperature and low salinity |
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| 中文摘要: |
| 以缢蛏Sinonovacula constricta(Lamarck)为研究对象，用双相电泳及图像分析等蛋白质组学技术探求其在高温、低盐2种生态压力下血淋巴内40KDa以下小分子碱性蛋白质的差异表达，以寻找缢蛏血淋巴的抗性机制，明确血淋巴在机体防御体系中的作用。在环境压力下，缢蛏血淋巴内有多种蛋白发生变化，MALDI-TOF—MS检测鉴定出磷脂酶A2、表皮分泌蛋白xp2前体、脑衍生神经营养因子、肿瘤坏死因子受体、脯氨酸顺反同工酶等7种蛋白或多肽。涉及多种与炎症反应、基质转运、神经营养、基因表达调控、蛋白质合成等有关的功能。说明其血淋巴在缢蛏的防御体系中具有关键性作用。 |
| English Summary: |
| Sinonovacula constricta(Lamarck), a kind of mollusks, is an important species of marine aquaculture. We choose it as our experiment sample and its basic protein expression profiles of hemolymph after short-term treatment ur(?)der high temperature and low salinity were detected, seven kinds of proteins or polypeptides were identified: Cysteine-rich protein 2 CRP2, Phospholipase A2, Pepidyl-prolyl cis- trans isomerase A, epidermal secretory protein xp2 precursor, brain-derived neurotrophic factor, tumor necrosis factor receptor, highmobility groupprotein HMGI-C. They have multiple functions, such as nutrition, gene expression regulation, protein synthesis, substances transport, antitoxin and anti-interference. The results suggested that the hemolymph played a key action in defense System of Sinonovacula constricta . |
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