| .扇贝裙边中糖胺聚糖的气相色谱定性定量分析及红外光谱分析[J].中国海洋药物,2004,23(6):8-11. |
| 扇贝裙边中糖胺聚糖的气相色谱定性定量分析及红外光谱分析 |
| Qualitative and quantitative analysis of glycosaminoglycan extracted from scallop skirts by gas chromatography and infrared spectrometry |
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| 中文关键词: 糖胺聚糖 提取分离 KB 定性定量分析 标准品 透明质酸 半乳糖 栉孔扇贝 海湾扇贝 吸收特征 |
| English Keywords:scallop,Chlamys farreri,glycosaminoglycan,neutral monosaccharides,gas chromatography,infrared spectrometry |
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| 中文摘要: |
| 目的 对栉孔扇贝和海湾扇贝裙边中提取分离的糖胺聚糖进行化学组成及结构研究。方法 样品经盐酸—甲醇试剂醇解成单糖．采用标准对照法及内标法进行气相色谱定性定量测定。样品经KBr压片，红外光谱扫描(4000～500cm^-1)。结果 测定出栉孔扇贝糖胺聚糖中单糖含量分别为鼠李糖0．75％，木糖0．63％，岩藻糖0．67％，甘露糖0．97％，葡萄糖1．11％，半乳糖1．59％。红外光谱测定结果显示，栉孔扇贝和海湾扇贝糖胺聚糖具有典型酸性黏多糖的吸收特征。结论 从栉孔扇贝裙边中提取分离的糖胺聚糖，分子中含有中性糖基。与标准品的红外光谱进行比较．两种扇贝糖胺聚糖均与透明质酸的红外光谱相似。 |
| English Summary: |
| Objective To study the chemical constituents and structures of the glycosaminogly-cans isolated from the leftover bits of the Chlamys farreri and Argopecten irradisus. Methods The qualitative and quantitative analyses of monosaccharides obtained by alcoholyses with HCl-methylalcohol from the samples were done by gas chromatography with standard contrast and inner standard methods. Mixed with KBr and pressed into pellet, the samples were analyzed by infrared spectrometer scanning from 4000-500 cm-1. Results The contents of monosaccharides from the samples were rhamnose 0. 75%, xylose 0. 63%, fucose 0. 67%, mannose 0. 97%, glucose 1. 11% and galactose 1. 59%, respectively. The infrared spectrum showed that the samples had typical infrared spectra of glycosaminoglycan. Conclusions The glycosaminoglycan isolated from the leftover bits of the Chlamys farreri contains neutral monosaccharides. Compared with standard glycosaminoglycans, the infrared spectra of the two samples are similar to that of hyaluronic acid. |
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