| .扇贝多肽对UVB辐射小鼠胸腺淋巴细胞的保护作用[J].中国海洋药物,2005,24(2):18-21. |
| 扇贝多肽对UVB辐射小鼠胸腺淋巴细胞的保护作用 |
| Protective effect of polypeptide from Chlamys farreri on UVB irradiated murine thymocytes in vitro |
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| DOI： |
| 中文关键词: 扇贝多肽 UVB 辐射保护 胸腺淋巴细胞 小鼠 凋亡 |
| English Keywords:Polypeptide from Chlamys farreri (PCF),UVB,radioprotection,thymocytes,mice,apoptosis |
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| 中文摘要: |
| 目的：研究扇贝多肽(polypeptide ftom Chlamys，farreri)对中波紫外线(UVB)辐射损伤小鼠胸腺淋巴细胞的保护作用。方法：UVB辐射小鼠胸腺淋巴细胞，MTT法检测胸腺淋巴细胞的活性；酶生化法检测细胞谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性；流式细胞术检测细胞内活性氧(ROS)的产生量以及细胞凋亡率。结果：PCF能明显减轻UVB辐射对小鼠胸腺淋巴细胞的损伤；提高细胞GSH-Px、SOD和CAT活性；降低细胞中ROS的产生量和细胞凋亡率。结论：PCF对小鼠胸腺淋巴细胞具有明显的辐射保护作用。 |
| English Summary: |
| Objective To study the radioprotective effect of polypeptide from Chlamys farreri (PCF) on ultraviolet B(UVB)-irradiated murine thmocytes in vitro. Methods The murine thymocytes were exposed to UVB radiation. MTT method was used to detect the cell viability. The activities of intracellular glutathione peroxidase (GSH-Px), superoxided dismutase(SOD) and catalase (CAT) were measured. Intracellular reactive oxygen species (ROS) and the effect of PCF on UVB-induced apoptosis were investigated by flow cytometry. Results PCF could greatly enhance the viability of murine thymocyte and markedly promote the activities of GSH-Px, SOD and CAT, while the amount of ROS was decreased. PCF could inhibit UVB-induced thymocyte apoptosis. Conclusion PCF has significant radioprotective effects on UVB-irradiated murine thymocytes in vitro. |
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