.昆布多糖的含量测定[J].中国海洋药物,2005,24(6):47-48. |
昆布多糖的含量测定 |
Determination of polysaccharide from Ecklonia kurome |
投稿时间:2005-03-10 |
DOI: |
中文关键词: 黑昆布 多糖 含量测定 |
English Keywords:Ecklonia kurome Okam polysaccharide determination |
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中文摘要: |
目的建立一种稳定、简便的昆布多糖含量测定方法。方法采用精制昆布多糖测得昆布多糖对葡萄糖的换算因子后,样品经除杂处理后,用苯酚-硫酸法测定昆布中多糖的含量。结果用此方法测定多糖的标准曲线,其相关系数为0.9998,平均回收率为97.35%,重现性相对标准偏差为4.68%。结论本方法所需仪器简单、操作方便,适合于昆布及其制品的多糖含量的常规检测。 |
English Summary: |
Objective To develop a stable and simple method to measure the polysaccharide in Ecklonia kurome Okam. Methods After the conversion coefficient of polysaccharide from Ecklonia kurome Okam to glucose was obtained, the content of polysaccharide was measured by phenol-sulfuric acid method. Results The correlation coefficient of standard curve was 0. 9998, the recovery rate was 97.35% and RSD was 4.68%. Conclusion The method was convenient for content determination of polysaccharide from Ecklonla kurorne Okam as well as its related products. |
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