| .硒化紫球藻胞外粗多糖的理化性质初步研究[J].中国海洋药物,2006,25(4):34-36. |
| 硒化紫球藻胞外粗多糖的理化性质初步研究 |
| Preliminary research of the physiochemical properties of selenium exopolysaccharide from Porphyridium sp. (Se-PSP) |
| 投稿时间：2006-11-09 |
| DOI： |
| 中文关键词: 硒,紫球藻,胞外多糖 |
| English Keywords:selenium,Porphyridium sp.,exopolysaccharide |
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| 中文摘要: |
| 目的研究硒化紫球藻胞外粗多糖的理化性质。方法在ASW培养基中加入适量的亚硒酸制备硒化紫球藻胞外多糖,并通过DEAE-52树脂分离纯化,Sephadex G200层析法鉴定其纯度;硫酸苯酚法测定硒化紫球藻多糖溶解度;pH计测量其溶液酸度;紫外可见分光光度法于260nm和280nm处分别定性测量核酸及蛋白质;纸层析法分离多糖;琼脂糖凝胶电泳测定溶液中糖带电情况。结果与结论培养过程中加入适量亚硒酸,对紫球藻生长没有显著性影响;制备的PSP及Se-PSP均不含蛋白质和核酸,显酸性、带负电荷;可能含有两个组分等。 |
| English Summary: |
| Objective To study the physiochemical properties of selenium exopolysaccharide from Porphyridium sp.(Se-PSP).Methods The Se-PSP was obtained from ASW,a sort of culture medium added with some selenious acid solution,and was purified by DEAE-52 cellulose column and identified by Sephadex G-200.The acidity,UV spectral absorption,paper chromatography,and gelose electrophoresis of Se-PSP solution were studied.Results and Conclusion Proper concentration of selenious acid in ASW culture had no influences upon the growth of Porphyridium.Se-PSP prepared in this way didn't contain protein and nucleic acid and the solution of Se-PSP showed acidity.It is probable that Se-PSP contain two fractions of PSP. |
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