海洋动物共附生微生物抗B16肿瘤细胞活性菌株的筛选

.海洋动物共附生微生物抗B16肿瘤细胞活性菌株的筛选[J].中国海洋药物,2006,25(6):26-30.

海洋动物共附生微生物抗B16肿瘤细胞活性菌株的筛选

Screening of cytotoxic activity of marine animal symbiotic and epiphyte microorganisms against B16 tumor cell

投稿时间：2005-06-28

DOI：

中文关键词: 海洋动物,海洋微生物,抗B16肿瘤细胞活性,统计分析

English Keywords:marine animal,marine microorganisms,cytotoxicity against B16 tumor cell,statistics analysis

Fund Project:

摘要点击次数: 2551

全文下载次数: 33

中文摘要:

目的分离具有小鼠黑色素瘤B16细胞毒活性海洋微生物。方法通过M1培养基、Gause培养基、Martin培养基分离单菌落,利用黄豆粉培养基发酵,目测法测定发酵液活性,利用SAS软件对结果统计分析。结果分离获得634株海洋微生物,筛选到活性菌株56株,活性菌株所占的比率为8.83%。结论从寄居蟹、毛螺、苔藓虫、三角涡虫、水螅、海绵分离筛选到的总菌数明显的高于其他动物,而从海葵、贻贝、文蛤、江瑶、海鞘、管虫、螃蟹分离到的活性菌株数高于其他动物。采样地点与分离到的微生物类别有显著相关性,各地点样品分离到的放线菌、细菌和真菌数占该地区分离到的菌株总数的比率最大的的地点依次是新村渔排、古城岭红树林和南湾港渔排;细菌、放线菌、真菌等不同微生物种类与活性菌株比率有显著相关性,细菌的活性菌株比率最高。

English Summary:

Objective To isolate marine microorganisms against murine melanoma cells B16.Methods Using Gause,M1,and Martin media for isolation.Isolates were fermented in the soy flour medium,the fermentation supernatant were tested against murine melanoma cells B16 by observing the growth inhibition or killing of the tumour cells under microscope.The results were statistically analyzed by SAS.Results Six hundred and thirty four strains of marine microorganisms were isolated from 101 marine animal samples,of which 56 active strains against murine melanoma cells B16 were screened out,the percentage of cytotoxic active strains was 8.83%.Conclusion The number of cytotoxic active strains was higher from Actina,Mytilus edulis,Meretrix meretrix,Atrina,Ascidiacea,Tube Worm,and crabs.There was significant relation between different microorganism types isolated to the sampling locations.Hainan Lingshui New Village fishing row had the highest percentage of the actinomycetes,GuangXi Beihai Guchengling mangrove had the highest percentage of bacteria,and GuangXi Beihai Nanwan habor had the highest percentage of fungi.There was significant relation between cytotoxic activity of the isolated strains to different microorganism types of bacteria,actinomycetes and fungi,among which thebacteria showed highest percentage of cytotoxic activity.

查看全文查看/发表评论下载PDF阅读器

关闭