| .毛蚶多糖的分离纯化和免疫活性测定[J].中国海洋药物,2007,26(2):23-26. |
| 毛蚶多糖的分离纯化和免疫活性测定 |
| Isolation,purification and immunological activity assay of polysaccharide from Arca subcrenata Lischke |
| 修订日期：2006-12-05 |
| DOI： |
| 中文关键词: 毛蚶 多糖 分离纯化 单糖组成 免疫活性 |
| English Keywords:Arca subcrenata Lischke polysaecharide purification neutral monosaccharide component immunological activity |
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| 中文摘要: |
| 目的从毛蚶体内分离纯化得到毛蚶多糖精品（polysaccharide from Arca subcrenata Lischke．简称ASLP），分析其纯度和单糖组成，同时考察免疫活性。方法经除蛋白、DEAE-52和Sephadex-GS0柱层析得多糖精品。糖醇乙酸酯法测定单糖组成。体外脾淋巴细胞增殖测定ASLP的免疫活性。结果从毛蚶体内提取到一种均一的多糖组分，其单糖组成只有葡萄糖，ASLP能够显著地促进脾淋巴细胞的增殖。结论从毛蚶中分离纯化得到一种均一的多糖组分，具有显著的体外免疫活性。 |
| English Summary: |
| Objective To isolate and purify a polysaccharide(ASLP)from Area subcrenata Lis- chke.Its purity,neutral monosaccharide component and immunological activity in vitro were determined.Methods The proteins were removed by Sevag method.The crude polysaccha- ride was purified by DEAE-52 anion-exchange chromatography and Sephadex G-50 gel filtra- tion chromatography.The neutral monosaccharide component was determined with alditol acetates method by GC.Lymphocyte proliferation assay was made to investigate the immu- no-modulating activity of ASLP.Results and Conclusion The results indicated ASLP was a homogeneous polysaccharide.It was a glucan with molecular weight of 35CODa.It could in- crease spleen lymphocyte proliferation remarkably.ASLP also showed significantly immuno- logical activity in vitro. |
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