| .疣缟芋螺毒素的二维液相色谱分离方法[J].中国海洋药物,2010,29(1):. |
| 疣缟芋螺毒素的二维液相色谱分离方法 |
| Conotoxin extraction and fractionation by the two-dimensional liquid chromatography from Conus lividus venom |
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| 中文关键词: 疣缟芋螺 二维液相色谱分离 芋螺毒素 |
| English Keywords:Conus lividus two-dimensional liquid chromatography conotoxin |
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| 中文摘要: |
| 目的建立一种新的基于二维液相色谱技术的疣缟芋螺毒素分离方法,了解其毒素组构成特点。方法从疣缟芋螺毒管中提取芋螺毒素总肽,在传统的凝胶色谱和反相色谱方法的基础上,根据芋螺毒素的等电点和疏水性,利用目标蛋白快速分离系统(Proteome Lab TMPF2D),对其进行二维分离。结果经过传统分离方法,能够检测得到40个左右的芋螺毒素条带,且分离效果不明显。而通过二维液相色谱分离方法,pI/UV图谱显示共检测到约200个芋螺毒素条带。结论同传统分离方法相比,采用Proteome LabTMPF2D系统对毒素分离更快速、分辨率更高,因此更利于鉴定芋螺毒素肽组分,也为下游序列结构特征与生物活性研究奠定了良好的基础。 |
| English Summary: |
| Objective To establish a separation protocol based on the two-dimensional liquid chromatography and to identify the conotoxin components from Conus lividus crude venoms.Methods The total crude venom was extracted from C.lividus venom ducts,which was fractionated by a novel two-dimensional liquid chromatography (ProteomeLabTMPF2D)after the initial gel filtration chromatography separation.Results There were only 40 conopeptide peaks detected when the traditional HPLC separation was applied for C.lividus crude... |
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