| 李冰,杨帆,吕丛仪,等.藻蓝蛋白联合全反式维甲酸诱导HeLa细胞凋亡的分子机制研究[J].中国海洋药物,2014,33(4):39-44. |
| 藻蓝蛋白联合全反式维甲酸诱导HeLa细胞凋亡的分子机制研究 |
| Molecular mechanism of facilitation of C-phycocyanin combined with all-transretinoic acid on the apoptosis of HeLa cells |
| 投稿时间：2014-02-28 修订日期：2014-03-18 |
| DOI： |
| 中文关键词: 全反式维甲酸 藻蓝蛋白 HeLa细胞 凋亡 增殖抑制 |
| English Keywords:All-transretinoic acid C-phycocyanin HeLa cells apoptosis Proliferation inhibition |
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| 中文摘要: |
| 目的 探讨全反式维甲酸和藻蓝蛋白联合用药对HeLa细胞生长的影响，并分析两者联合用药诱导细胞凋亡可能的分子机制。方法 MTT法检测全反式维甲酸和藻蓝蛋白单独及联合用药对HeLa细胞生长的影响，TUNEL法检测单独及联合用药HeLa细胞凋亡情况，免疫组化法检测Bcl-2基因的表达情况，Westren blot法检测Caspase-3的表达情况。结果 全反式维甲酸和藻蓝蛋白均具有抑制HeLa细胞生长的作用，当达到相同的抑制率时，联合藻蓝蛋白使用可以显著降低全反式维甲酸的使用剂量从而达到降低毒副作用的目的。2种药物联合用药处于中高抑制率时，2种药物为协同作用。两者联合用药可以显著下调Bcl-2和增加Caspase-3的表达水平从而引发细胞凋亡。结论 2种药物单独使用均能抑制HeLa细胞的生长，联合用药时该抑制作用更为显著。全反式维甲酸和藻蓝蛋白联合用药诱导HeLa细胞凋亡的分子机制可能是通过抑制bcl-2基因的表达、促进Caspase-3蛋白的表达，从而促进凋亡信号的转导最终导致细胞死亡的。
关键词：全反式维甲酸；藻蓝蛋白；HeLa细胞；凋亡；增殖抑制 |
| English Summary: |
| Objective We focused on studying the effects of All-transretinoic acid (ATRA), C-phycocyanin (C-PC) or ATRA C-PC on HeLa cells growth and apoptosis. Methods MTT assay was adopted to determine the effects of C-PC and ATRA on the growth of HeLa cells. TUNEL assay was adopted to determine the levels of cellular apoptosis. The expression quantities of Caspase-3 and Bcl-2 were determined by Western blot and immunohistochemistry staining. Results Both C-PC and ATRA could inhibit the growth of HeLa cells. The dosage of ATRA was reduced when it cooperated with C-PC. When the combined therapy of two drugs are in a high inhibition rate, the two drugs are synergy. ATRA treated with C-PC could induce more cells apoptosis by down-regulation of Bcl-2 gene and up-regulation of Caspase-3 gene. Conclusion Single drug could inhibit HeLa cells growth and the combined drugs had an enhanced inhibitory effect. C-PC ATRA combination might induce the apoptosis of HeLa cells by down-regulating Bcl-2 gene, up-regulating Caspase-3 gene and inducing the transduction of apoptotic signals. |
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