| 栾晓红,赵峡,王清池.缢蛏多糖的提取、分离和结构分析[J].中国海洋药物,2015,34(2):22-28. |
| 缢蛏多糖的提取、分离和结构分析 |
| Extraction, separation and structural characterization of polysaccharides from Sinonovacula Constricta |
| 投稿时间：2014-10-08 修订日期：2014-11-16 |
| DOI： |
| 中文关键词: 缢蛏 多糖 提取 分离 结构分析 |
| English Keywords:Sinonovacula Constricta polysaccharide extraction separation structural characterization |
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| 中文摘要: |
| 目的 从缢蛏中提取和分离纯化多糖,并对其基本理化性质和结构组成进行分析。方法 依次采用100 ℃热水提和碱提方法从缢蛏中提取粗多糖,采用Q Sepharose Fast Flow强阴离子交换柱层析和Sephacryl S-300凝胶柱层析对粗多糖进行分离纯化,并对其总糖、蛋白含量,相对分子质量和单糖组成进行分析。对多糖纯化组分采用甲基化、气质联用(GC-MS)、红外光谱(IR)和核磁共振波谱(NMR)对其化学结构进行解析。结果 从缢蛏中经热水提粗多糖(YC-S)和碱提粗多糖(YC-J)均为葡聚糖,进一步分离纯化得到了4种水溶性多糖组分。对其中1种热水提多糖纯化组分YC-S2采用高效液相凝胶色谱法测得其相对分子质量为482 kD,且色谱峰单一对称,表明其具有较高的纯度。结构分析表明,YC-S2是一种以α-(1→4)Glc为主链,含有少量的β-(1→3,4)和β-(1→4)分支的D-吡喃型葡聚糖。结论 从缢蛏中提取分离得到了4种多糖组分,并初步确定了一种水溶性葡聚糖的结构,为缢蛏多糖结构和活性的深入研究提供了基础。 |
| English Summary: |
| Objective To extract and purify polysaccharides from Sinonovacula Constricta, and investigate their physical and chemical properties and structural characteristics. Methods Sinonovacula Constricta polysaccharides were extracted by distilled water at 100 ℃ and alkali solution sequentially, and purified by Q sepharose Fast Flow anion-exchange and Sepharcryl S-300 gel-permeation chromatography. The contents of total sugar, protein and sulfate were determined by various chemical methods, and monosaccharide compositions were analyzed by high performance liquid chromatography. The structure of polysaccharides was characterized by methylation analysis, IR, GC/MS and NMR techniques. Results Four polysaccharides were isolated from Sinonovacula Constricta. The water-extracted polysaccharides (YC-S) and alkali solution-extracted polysaccharides (YC-J) are soluble glucans. The relative molecular weight of YC-S2 determined by high performance liquid chromatography is 482 kDa. The structure of YC-S2 was characterized to be α-(1→4)-D-glucopyranose, with a little β-(1→3,4) and β-(1→4) branched units. Conclusion Four polysaccharides fractions were separated from Sinonovacula Constricta and the structure of a water-soluble polysaccharide (YC-S2) was determined. These data provide a basis for further research on relationships of structure and activity of Sinonovacula Constricta polysaccharides. |
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