| 张丹,蒋群,李英新,等.中国南海海绵和珊瑚共附生真菌多样性及其抑菌活性研究[J].中国海洋药物,2015,34(2):1-9. |
| 中国南海海绵和珊瑚共附生真菌多样性及其抑菌活性研究 |
| Diversity and antibacterial activity of fungi associated with South China Sea sponges and corals |
| 投稿时间：2014-10-30 修订日期：2014-11-04 |
| DOI： |
| 中文关键词: 海绵 珊瑚 真菌多样性 抑菌活性 |
| English Keywords:Sponge Coral Fungi diversity Antibacterial activity |
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| 中文摘要: |
| 目的 揭示中国南海海绵和珊瑚共附生真菌多样性及其抗菌活性。方法 采用9种不同的真菌培养基对海绵和珊瑚中的共生真菌进行分离培养和纯化,根据真菌18S rDNA对所分离的真菌进行鉴定,使用MEGA 5.2软件对所分离真菌进行系统发育分析。采用圆形滤纸片法进行抑菌活性筛选。结果 从采自南海的6种海绵与3种珊瑚中共分离得到106株真菌菌株,通过形态分析和序列分析排重得到41株独立真菌菌株。18S rDNA序列比对结果分析显示,分别属于子囊菌门(Ascomycota)的11个目和担子菌门(Basidiomycota)的4个目,共计26个属。其中子囊菌门所占比例最高为93.4%,剩下的6.6%为担子菌门。通过抑菌活性筛选显示部分真菌对革兰氏阳性细菌和革兰氏阴性细菌具有广谱抗菌活性。结论 本研究揭示了中国南海海绵和珊瑚共附生真菌丰富的种群多样性及部分真菌的抑菌活性,为进一步开发利用海绵和珊瑚共附生真菌及生产活性物质奠定了基础。 |
| English Summary: |
| Objective To reveal the diversity and antibacterial activity of fungi isolated from sponges and corals in the South China Sea. Methods Nine kinds of fungal media were used to isolate and purify the fungi from sponges and corals. The fungi were identified through fungi 18S rDNA by BLAST tool. Phylogenetic tree was conducted by MEGA 5.2. Antibacterial activity was tested using standard disk diffusion. Results A total of 106 fungi were isolated from six sponge species and three coral species. A total of 41 independent isolates, which belong to 26 genera within 11 orders of Ascomycota and 4 orders of Basidiomycota, were identified by sequences analysis. The fungi from Ascomycota were the most dominant, occupying 93.4% of the fungal isolates, while the rest belonged to Basidiomycota. The antibacterial activity test indicated that several fungi were active against gram-positive and gram-negative bacteria. Conclusion The results showed high diversity and antibacterial activities of fungi derived from sponges and corals, which laid the basis for the further utilization of fungi associated with sponges and corals for marine drugs development. |
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