| 曲承蕾,杨雪,张淑敏,等.海洋源放线菌Streptomyces sp. 223中二酮哌嗪类成分的研究[J].中国海洋药物,2015,34(3):23-28. |
| 海洋源放线菌Streptomyces sp. 223中二酮哌嗪类成分的研究 |
| Study of piperazinedione derivatives from the marine-derived Streptomyces sp. 223 |
| 投稿时间：2014-11-04 修订日期：2015-01-06 |
| DOI： |
| 中文关键词: 海洋放线菌 次级代谢产物 二酮哌嗪类化合物 结构鉴定 细胞毒活性 |
| English Keywords:Marine actinomycetes secondary metabolites piperazinedione structure elucidation cytotoxic activity |
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| 中文摘要: |
| 目的 对采集自烟台逛荡河入海口沉积物的一株放线菌Streptomyces sp. 223进行次级代谢产物及活性研究。方法 采用硅胶柱层析、Sephadex LH-20凝胶层析、薄层层析以及HPLC等方法对放线菌Streptomyces sp. 223发酵产物进行分离纯化,通过波谱数据分析及文献比较对化合物进行结构鉴定,并用MTT法初步评价其细胞毒活性。结果 从该菌的发酵产物中分离到了7个二酮哌嗪类化合物,分别鉴定为(3S)-(1,4)-二甲基-3-异丙基-(2,5)哌嗪二酮(1),环-(脯-苯丙)二肽(2),环(脯-酪)二肽(3),环(4-OH-脯-亮)二肽(4),环(苯丙-甘)二肽(5),环(4-OH-脯-苯丙)二肽(6)和环-(脯-缬)二肽(7)。结论 化合物1为首次从海洋微生物中分离得到的新颖天然产物,且具有显著的细胞毒活性,对Hela细胞的半抑制浓度IC50=18.7 μmol.L-1。 |
| English Summary: |
| Objective To investigate the bioactive secondary metabolites from the marine-derived actinomycete Streptomyces sp. 223 isolated from a estuary sediment of Guangdang River, Yantai. Methods The fermentation products were separated via silica gel column chromatography, Sephadex LH-20, thin layer chromatography and HPLC. The structures of the purified compounds were elucidated on the basis of spectral analysis and comparison with the data in literatures. The cytotoxicities were determined by MTT method. Results Totally 7 compounds were separated and elucidated as (3S)-1,4-dimethyl-3-(1-methylethyl)- 2,5-piperazinedione (1), cyclo-(Pro-Phe) (2), cyclo-(Pro-Tyr) (3), cyclo-(4-hydroxyl-Pro-Leu) (4), cyclo-(Phe-Gly) (5), cyclo-(4-hydroxyl-Pro-Phe) (6), and cyclo-(Pro-Val) (7). Conclusion compound 1 was firstly isolated from marine actinomyces as a new natural product and showed cytotoxicities against Hela cell line with IC50 =18.7 μmol.L-1. |
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