| 谢尚举.复合rhBMP-2壳聚糖水凝胶的制备及其缓释性能研究[J].中国海洋药物,2015,34(4):31-36. |
| 复合rhBMP-2壳聚糖水凝胶的制备及其缓释性能研究 |
| PREPARATION OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN 2 LOADED CHITOSAN HYDROGEL AND ITS RELEASE PROPERTY RESEARCH |
| 投稿时间：2014-12-12 修订日期：2015-03-28 |
| DOI： |
| 中文关键词: 重组人骨形态发生蛋白2 壳聚糖 水凝胶 缓释 |
| English Keywords:Recombinant human bone morphogenetic protein 2 Chitosan Hydrogel Sustained-release |
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| 中文摘要: |
| 【】目的 通过构建新型负载重组人骨形态发生蛋白2(Recombinant Human Bone Morphogenetic Protein 2 rhBMP-2)壳聚糖水凝胶缓释系统来获得持续稳定释放的rhBMP-2细胞因子。方法 分别用1.0mg/ml、1.5mg/ml、2.0mg/ml壳聚糖(Chitosan CS)和0.5mg/ml、0.7mg/ml、1.0mg/ml三聚磷酸钠(Sodium Tripolyphosphate TPP)按总质量比5:1交联,比较各组溶胀系数,扫描电镜(SEM)观察各组凝胶微观表面形态,采用最佳溶胀度组水凝胶复合rhBMP-2,酶联免疫吸附试剂盒(ELISA)动态检测负载rhBMP-2壳聚糖水凝胶的缓释特性。结果 1.5mg/ml CS与0.5mg/ml TPP交联所得水凝胶表现出最低溶胀度,为238.28?Ee28.06%,该组溶胀度与其他各组比较,差异具有统计学意义(P﹤0.05)。其冻干水凝胶扫描电镜下呈三维网状结构,内部均匀分布壳聚糖微球,将其负载rhBMP-2后获得体外释放曲线如下。结论 新型rhBMP-2壳聚糖水凝胶制备流程简单,且具有很好的承载和缓释rhBMP-2的能力,为rhBMP-2在骨组织工程中的应用提供了良好的新途径。 |
| English Summary: |
| 【】 Objective To develop and characterize a novel chitosan hydrogel delivery system for obtaining a sustained-release of recombinant human bone morphogenetic protein 2 (rhBMP-2). Methods The blank chitosan hydrogels were prepared by using 1.0mg/ml, 1.5mg/ml, 2.0mg/ml chitosan (CS) respectively cross-linked with 0.5mg/ml, 0.7 mg/ml, 1.0mg/ml sodium tripolyphosphate (TPP), and the total mass ratio of CS: TPP is 5:1. Then determined and compared the swelling degree of each group, afterward, observed microscopic morphology of chitosan hydrogels in each group by scanning electron microscopy (SEM), at last, used the group of hydrogel which had optical swelling degree composited with rhBMP-2. Finally, dynamically detected rhBMP-2 chitosan hydrogel’s sustained-release characteristic by enzyme-linked immunosorbent assay kit (ELISA). Results Chitosan hydrogel which cross-linked by 1.5mg/ml CS with 0.5mg/ml TPP showed the lowest swelling degree, the swelling degree was 238.28% ± 28.06%, when compared with other groups, the difference was statistically significant (P <0.05). Then the freeze-dried chitosan hydrogel was visible under SEM with three-dimensional network structure, furthermore, there uniformly distributed with chitosan microspheres in gel internal, and the rhBMP-2 in vitro release was determined as fellows. Conclusion The novel rhBMP-2-chitosan hydrogel possessed simple preparation process and splendid carrying and releasing capacity on rhBMP-2, which provided a new way for rhBMP-2 in bone tissue engineering applications. |
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