| 曹秀明,樊宇,宋冬雪,等.虾青素促进丙烯酰胺所致大鼠海马神经细胞损伤的恢复[J].中国海洋药物,2015,34(3):9-13. |
| 虾青素促进丙烯酰胺所致大鼠海马神经细胞损伤的恢复 |
| Astaxanthin Promotes Acylamide- damaged Hippocampal Neurons to Restore Damage |
| 投稿时间：2015-01-04 修订日期：2015-02-12 |
| DOI： |
| 中文关键词: 虾青素 丙烯酰胺 海马神经细胞 氧化损伤 |
| English Keywords:Astaxanthin, acrylamide, hippocampal neurons, oxidative damage |
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| 中文摘要: |
| 目的 从细胞水平上研究虾青素对丙烯酰胺所致海马神经细胞损伤恢复的影响。方法 通过B27无血清培养法进行SD乳鼠的大脑海马神经细胞的分离、培养及其鉴定；MTT法检测细胞存活率,比色法测定细胞内抗氧化酶SOD、CAT、GSH-Px的活性,线粒体的MDA及NO的含量。结果 虾青素能提高丙烯酰胺损伤过的海马神经细胞的存活率,提高了丙烯酰胺损伤后细胞SOD、CAT活性、GSH-Px的活力,并降低了细胞线粒体中的MDA和NO的含量。结论 虾青素对丙烯酰胺造成的高海马神经细胞损伤有促进恢复的作用。 |
| English Summary: |
| Objective To investigate the effects of Astaxanthin on the acylamide-damaged Hippocampal Neurons. Methods The Hippocampal Neuron isolated from SD neonatal rats,cultured by B27 serum-free medium and identified by NSE immunohistochemistry assay. MTT assay was used to examine the cell viability. The activity of SOD, CAT, GSH-Px was determined with Colorimetric methods as well as the content of MDA and NO of chondriosome in the Hippocampal Neuron. Results Astaxanthin improves the cell viability of acyamide-damaged Hippocampal Neurons, and the activity of SOD, CAT and GSH-Px as well as reduces the content of MDA and NO of chondriosome. Conclusion: Astaxanthin promotes Acylamide- damaged Hippocampal Neurons to Restore Damage. |
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