赵红玲,李松涛,王小青,等.蓝贻贝抗凝肽的合成及活性初步研究Δ[J].中国海洋药物,2015,34(5):59-62.
蓝贻贝抗凝肽的合成及活性初步研究Δ
Blue mussel anticoagulant peptide synthesis and preliminary research activity
投稿时间:2015-01-09  修订日期:2015-05-28
DOI:
中文关键词:  蓝贻贝  抗凝肽  固相合成  抗凝活性
English Keywords:blue mussel  anticoagulant peptide  Solid-phase synthesis  Anticoagulant activity
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作者单位E-mail
赵红玲 河北省中药研究与开发重点实验室 zhldxy@163.com 
李松涛 河北省中药研究与开发重点实验室  
王小青 河北省中药研究与开发重点实验室  
杨鹤松 河北省中药研究与开发重点实验室  
郝婷 河北省中药研究与开发重点实验室  
夏丹 苏州中科天马肽工程有限公司  
尹志峰 河北省中药研究与开发重点实验室  
王良友** 河北省中药研究与开发重点实验室 liangywang@gmail.com 
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中文摘要:
      目的采用多肽固相合成法合成蓝贻贝抗凝肽MEAP(Blue mussel Mytilus edulis anticoagulant peptide),初步研究其抗凝活性。方法 以2-CTC树脂为载体, Fmoc保护氨基酸为原料,按照蓝贻贝抗凝肽的序列从C端向N端逐步偶联氨基酸,裂解后得到粗肽,经半制备液相纯化后测定精肽的凝血酶时间(TT)、活化的部分凝血活酶时间(APTT)、凝血酶原时间(PT)。结果 合成的蓝贻贝抗凝肽粗品的纯度为65.5%,经半制备纯化后的精肽纯度为95.5%;抗凝活性实验显示APTT、TT具有显著的延时作用(P<0.01)。结论 固相合成的蓝贻贝抗凝肽具有抗凝活性。
English Summary:
      Purpose To prepare blue mussel Mytilus edulis anticoagulant peptide (MEAP) by using solid phase peptide synthesis method and to preliminary study its anticoagulant activity. Methods MEAP was successfully synthesized from its C terminal to N terminal according to its peptide sequence by using 2-CTC Resin as the solid supporter and Fmoc-amino acids as raw materials. The crude peptide was cleaved from the resin and purified by semi-prepare RP-HPLC. The thrombin time (TT), activation of blood coagulation time (APTT) and prothrombin time (PT) of purified MEAP were determined. Results The purity of synthetic crude and purified MEAP was 65.5% and 95.5% respectively. Anticoagulant activity experiments showed that both APTT and TT of synthetic MEAP had significant delayed effect (P < 0.01). Conclusion MEAP by using solid phase peptide synthesis method has anticoagulant activity.
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