| 刘贵娟,李卓悦,石杰,等.羧甲基壳寡糖对阿尔茨海默病模型大鼠的作用研究[J].中国海洋药物,2015,34(5):26-32. |
| 羧甲基壳寡糖对阿尔茨海默病模型大鼠的作用研究 |
| Study on the carboxymethyl chitosan oligosaccharide effect of rats models with Alzheimer's disease |
| 投稿时间：2015-03-10 修订日期：2015-04-20 |
| DOI： |
| 中文关键词: 阿尔茨海默氏病 羧甲基壳寡糖 β-淀粉样蛋白 |
| English Keywords:Alzheimer |
| Fund Project: |
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| 中文摘要: |
| 目的 探讨不同剂量的羧甲基壳寡糖对阿尔茨海默病（Alzheimer's disease，AD）模型大鼠的作用效果及机制。方法 通过侧脑室注射β-淀粉样蛋白（Aβ25-35）建立老年痴呆症大鼠模型，以羧甲基壳寡糖（CM-COS）高剂量（250mg?kg?1?d ?1）和低剂量（150mg?kg?1?d ?1）对模型组大鼠进行连续4周的灌胃治疗，通过Morris水迷宫实验检测大鼠的学习记忆能力，通过测定大鼠海马组织中的MDA、A-CHE、SOD、CHAT及GSH-PX的含量，研究CM-COS对AD大鼠的作用效果及机制。结果 CM-COS高低剂量组均能明显缩短大鼠逃避潜伏期的时间，增加其穿越平台的次数，均能明显降低大鼠大脑海马区MDA和A-CHE的含量，显著提高SOD、CHAT及GSH-PX的含量。结论 CM-COS通过提高AD大鼠海马组织的抗氧化能力和改善其胆碱能系统，从而改善AD大鼠的学习记忆能力，对Aβ25-35所致的老年痴呆症有一定的改善作用。 |
| English Summary: |
| Objective To investigate the effect and mechanism of carboxymethyl chitosan oligosaccharide on Alzheimer?s disease model rats. Methods Aβ25-35was injected into the rats lateral ventricle to make AD models. Using the different doses of carboxymethyl chitosan oligosaccharide (CM-COS) respectively，the model group rats were intragastric administration therapy. After intragastric administration for 4 weeks,the learning and memory ability of rats were tested with Morris water labyrinth. To determine the role of CM-COS on the effect and mechanism of AD rat, the content of MDA, A-CHE, SOD, CHAT and GSH-PX in the rats hippocampal tissue were measured. Results CM-COS could significantly decrease the content of MDA and A-CHE in hippocampus of animal models and could significantly increase the content of SOD, CHAT and GSH-PX. Morris water labyrinth experiment results showed that carboxymethyl chitosan oligosaccharide could significantly shorten the animal escape latency time and increase the number of cross platform. Conclusion CM-COS in hippocampal tissue could improvetheability of learning and memory in AD rats by increasing the antioxidant capacity and improving the cholinergic system,and had a certain therapeutic effect on Aβ25-35 caused by Alzheimer's disease. |
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