| 赵海婷,张秀娟,曲桂武,等.青环海蛇抗菌肽改造体凝胶剂有效成分的含量测定[J].中国海洋药物,2015,34(5):81-85. |
| 青环海蛇抗菌肽改造体凝胶剂有效成分的含量测定 |
| Content determination of effective constituent in body transformation Gels of Hydrophis cyanocinctus Antimicrobial Peptide |
| 投稿时间：2015-03-23 修订日期：2015-04-30 |
| DOI： |
| 中文关键词: 青环海蛇抗菌肽改造体凝胶剂 有效成分 高效液相色谱法 含量测定 |
| English Keywords:body transformation Gels of Hydrophis cyanocinctus Antimicrobial Peptide effective constituent HPLC Content determination |
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| 中文摘要: |
| 摘 要:目的 建立抗菌肽HC-17凝胶剂有效成分的含量测定方法。方法 采用高效液相色谱法测定有效成分的含量，以乙腈：0.1%三氟乙酸为流动相进行梯度洗脱，洗脱条件0~25 min (23:77-48:52)，流速1.0 mL·min-1，检测波长220 nm，柱温35℃。结果 凝胶剂的有效成分抗菌肽HC-17在0.20~1.40 mg·mL-1浓度范围内线性关系良好（r=0.9995），平均加样回收率为101.47%，RSD=1.35%。结论 建立的方法准确、稳定、重现性好，可用于抗菌肽HC-17凝胶剂有效成分的定量分析。 |
| English Summary: |
| Abstract:Objective Content determination method was established to investigate the effective constituent in antimicrobial peptide HC-17 Gels. Methods The content of active principle in antimicrobial peptide HC-17 was determined by high performance liquid chromatography (HPLC) ,the mobile phase was acetonitrile-0.1% trifluoroacetic acid with the flow rate of 1.0 mL·min-1, the detection wavelength was 220 nm, and the column temperature was set at 35℃. Results The linear range of antibacterial peptide HC - 17 was 0.20~1.40 mg·mL-1 (r=0.9995) with an average recovery of 101.47%, RSD was 1.35%. Conclusion The developed method was accurate, stabilize, reproducible and which can be used for the quantify analysis of active principle in antimicrobial peptide HC-17 gels . |
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