| 薛金凤,李文婷,陆园园,等.南极放线菌XE发酵条件的优化及抑菌物质性质的初步研究[J].中国海洋药物,2016,35(2):23-29. |
| 南极放线菌XE发酵条件的优化及抑菌物质性质的初步研究 |
| Fermentation optimization of XE from Antarctic actinomycetes and the study of antimicrobial active substance |
| 投稿时间：2015-05-24 修订日期：2015-07-21 |
| DOI： |
| 中文关键词: 放线菌XE 发酵优化 极地放线菌 抑菌物质 |
| English Keywords:Actinomycetes XE fermentation optimization Polar-derived actinomycetes antibacterial substances |
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| 中文摘要: |
| 目的 优化南极放线菌XE发酵工艺,提高抑菌活性物质的产量并对菌株产生的活性代谢产物性质进行了初步研究。方法 以发酵液抑菌活性为指标,采用单因素实验和正交实验对放线菌XE发酵培养基和发酵条件进行优化；观察pH值、温度、储藏温度对抑菌物质稳定性的影响；了解抑菌物质的极性及疏水特性。结果 获得了南极放线菌XE的最佳发酵培养基：可溶性淀粉30g/L,黄豆10g/L,海水晶35g/L,KON3 1.5g/L；最佳发酵条件：温度28℃,起始pH8,接种量2%,摇床转速110rpm,发酵时间3天；抑菌物质热稳定性好,-20℃储藏活性基本不变,在强碱环境中失活,不耐强碱,活性物质极性较小。结论 通过优化工艺实验,确定了菌株XE最佳发酵培养基和发酵条件,抑菌物质热稳定性好,在-20℃储藏活性基本不变,极性较小,不耐强碱。 |
| English Summary: |
| Objective To optimize the fermentation process with the aim of improving the antibacterial substance in the Streptomyces sp. XE secondary metabolite, and to study the characteristics of active substances secreted by strain XE. Methods Single-factor experiment and orthogonal tests were used to optimize the fermentation medium and conditions. The effect of pH, temperature and storage temperature on the stability of active substances was investigated to study the hydrophobic nature of the antibacterial substances. Results The optimal fermentation medium:soluble starch 30 g/L, soybean 10g/L, sea crystal 35g/L, KNO3 1.5 g/L; the optimal fermentation conditions: temperature 28℃, starting pH 8, inoculum concentration 2%, shaking speed 110rpm, fermentation time 3 days. The active substances exhibit stability against heat and the activity is essentially the same under -20℃ storage, but the active substance possess less polarity and lose activity under strong alkali environment. Conclusion The optimal fermentation medium and conditions were obtained and the active substances possess the great stability against heat and under -20℃ storage, less polarity. It exhibited antibacterial activity at pH 2~12, whereas it was inactive under high alkalic condition. |
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