| 陈亮亮,王佩,王昊,等.角果木内生真菌Coriolopsis sp. J5代谢产物及活性研究[J].中国海洋药物,2016,35(2):7-12. |
| 角果木内生真菌Coriolopsis sp. J5代谢产物及活性研究 |
| Study on the secondary metabolites from the endophytic fungus Coriolopsis sp. J5 of Ceriops tagal and their biological activities |
| 投稿时间：2015-09-24 修订日期：2015-11-02 |
| DOI： |
| 中文关键词: 角果木 内生真菌 化学成分 细胞毒活性 抗菌活性 |
| English Keywords:Ceriops tagal endophytic fungus chemical constituent cytotoxic activity antibacterial activity |
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| 中文摘要: |
| 摘 要：目的 研究红树植物角果木内生真菌Coriolopsis sp. J5的活性次生代谢产物。方法 采用多种柱色谱技术对次生代谢产物进行分离纯化，根据理化常数和波谱数据分析鉴定化合物的结构；分别采用MTT法和滤纸片琼脂扩散法测定化合物的体外细胞毒活性及抗菌活性。结果 从角果木内生真菌Coriolopsis sp. J5的次生代谢产物中分离鉴定了7个化合物，分别为14-acetoxy-15-hydroxyirpexan（1）、15α-hydroxytrametenolic acid（2）、3β-hydroxylanosta-8,24-
dien-21-oic acid（3）、latifolicinin C（4）、对羟基苯乙酸甲酯（5）、methyl 5-（2-methoxycarbonylethy）furan-2-carboxylate（6）和5-(2-carboxy-ethyl)-furan-2-carboxylic acid（7）。化合物1对人慢性髓原白血病细胞(K-562)和人肝癌细胞（BEL-7420）生长具有抑制作用，化合物5具有抗白色念珠菌（Candida albicans）、棉花黄萎病菌（Verticillium dahliae Kleb）和棉花枯萎镰刀菌（Fusarium oxysporum f.sp. vasinfectum）活性，化合物6具有抗地毯草黄单胞菌（Xanthomonas axonopodis）活性。结论 化合物1~5为首次从角果木内生真菌的次级代谢产物中分离得到，化合物6和7为新天然产物，并首次报道了化合物7的核磁数据；菌株Coriolopsis sp. J5能代谢产生具有细胞毒活性和抗菌活性的化合物。 |
| English Summary: |
| Abstract: Objective To study the bioactive constituents from endophytic fungus Coriolopsis sp. J5 of Ceriops tagal. Methods The secondary metabolites were isolated and purified by various column chromatography techniques, and their structures were identified through a combined analysis of physicochemical properties and spectroscopic evidence. The antibacterial and cytotoxic activities in vitro of the compounds were evaluated by paper disco diffusion method and MTT method, respectively. Results Seven compounds were isolated from marine fungus Coriolopsis sp. J5. Their structures were determined as 14-acetoxy-15-hydroxyirpexan (1), 15α-hydroxytrametenolic acid (2), 3β-hydroxylanosta-8,24-dien-21-oic acid (3), latifolicinin C (4), methyl-p-hydroxphenylacetate (5), methyl 5-(2-methoxycarbonylethy)furan-2-carboxylate (6) and 5-(2-carboxy-ethyl)-furan-2-carboxylic acid (7). Bioassay results showed that compound 1 exhibited cytotoxic activity against K-562 and BEL-7420 tumor cell lines. Furthermore, compound 5 showed inhibitory effect on Candida albicans, Verticillium dahliae Kleb and Fusarium oxysporum f.sp. vasinfectum, and compound 6 showed inhibitory effect on Xanthomonas axonopodis. Conclusion Compounds 1~5 were isolated from Ceriopstaga genus for the first time. Moreover, the compounds 6 and 7 were new natural products, and NMR data of compound 7 were reported for the first time. In addition, the endophytic fungus Coriolopsis sp. J5 could produced compounds with antimicrobial and cytotoxic activities. |
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