| 肖波,杨宝勤,张其,等.红树林放线菌Streptomyces sp.CHQ-61产生的胞外多糖结构及自由基清除活性[J].中国海洋药物,2016,35(4):6-10. |
| 红树林放线菌Streptomyces sp.CHQ-61产生的胞外多糖结构及自由基清除活性 |
| Structure and free radical scavenging activity of an exopolysaccharide produced by the mangrove actinomycetes Streptomyces sp.CHQ-61 |
| 投稿时间：2016-01-05 修订日期：2016-01-22 |
| DOI： |
| 中文关键词: 红树林放线菌 胞外多糖 结构特征 自由基清除活性 |
| English Keywords:mangrove actinomycetes exopolysaccharide structural characterization free radical scavenging |
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| 中文摘要: |
| 目的 探究红树林放线菌Streptomyces sp.CHQ-61产生的胞外多糖的结构及自由基清除活性。方法 利用乙醇沉淀法、阴离子交换层析柱和凝胶层析柱对菌株发酵产物中的胞外多糖进行分离纯化,运用核磁共振、气质联用色谱、红外光谱、高效凝胶渗透色谱和柱前衍生高效液相色谱分析鉴定多糖的结构特征,采用DPPH、超氧阴离子和羟基自由基清除率评价胞外多糖的抗氧化活性。结果 从红树林放线菌Streptomyces sp.CHQ-61发酵产物中分离得到多糖XH-1,其分子量为8.9 kDa,丙酮酸含量为4.0 %,主要由半乳糖和氨基葡萄糖组成,糖链主要含有(1→6)Galp,还有少量的(1→3)GlcNp、(1→4,6)Galp、(1→3,6)GlcNp、末端Galp和GlcNp,XH-1具有一定的体外自由基清除活性,其清除DPPH、超氧阴离子和羟基自由基的EC50分别为0.5、0.8和3.0 mg/mL。结论 本文首次报道了红树林根泥放线菌Streptomyces sp.CHQ-61产生的胞外多糖XH-1为1种含有丙酮酸的半乳糖和氨基葡萄糖组成的结构新颖的胞外多糖,其具有较强的体外自由基清除活性。 |
| English Summary: |
| Objective To investigate the structure and free radical scavenging activity of the exopolysaccharide produced by the mangrove actinomycetes Streptomyces sp.CHQ-61. Methods An exopolysaccharide was isolated from the culture medium of the mangrove actinomycetes Streptomyces sp.CHQ-61 using ethanol precipitation, anion-exchange and gel-permeation chromatography. The structural characteristics of the exopolysaccharide was identified by HPGPC, PMP-HPLC, infrared spectrum, methylation analysis and NMR. The free radical scavenging activity was evaluated by the scavenging abilities on DPPH, superoxide and hydroxyl residues in vitro. Results The exopolysaccharide XH-1 was obtained from the fermented broth of the mangrove actinomycetes Streptomyces sp.CHQ-61. The molecular weight of XH-1 was estimated as 8.9 kDa and its pyruvic acid content was 4.0 %. XH-1 was mainly composed of galactose with small amounts of glucosamine. The chain of XH-1 mainly consisted of (1→6)Galp, with minor amounts of (1→3)GlcNp, (1→4,6)Galp, (1→3,6)GlcNp, (1→Galp and (1→GlcNp. XH-1 had a good scavenging activity of free radicals. EC50 values of XH-1 for DPPH, superoxide and hydroxyl radicals were 0.5 mg/mL, 0.8 mg/mL and 3.0 mg/mL, respectively. Conclusion XH-1 from the mangrove actinomycetes Streptomyces sp.CHQ-61 was a exopolysaccharide with novel structure which was composed of galactose and glucosamine. XH-1 has a high scavenging activity on free radicals in vitro. |
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