| 张君,任鹏飞,田黎,等.海洋芽孢杆菌B-9987中抗MDR菌活性成分研究[J].中国海洋药物,2016,35(5):45-49. |
| 海洋芽孢杆菌B-9987中抗MDR菌活性成分研究 |
| Identification of anti-MDRB compounds from the marine-derived Bacillus sp. B-9987 |
| 投稿时间：2016-01-23 修订日期：2016-02-26 |
| DOI： |
| 中文关键词: 海洋芽孢杆菌B-9987 多重耐药菌 Macrolactin Bacillaene |
| English Keywords:Marine Bacillus sp. B-9987 multi-drug resistant bacteria (MDRB) Macrolactin Bacillaene |
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| 中文摘要: |
| 挖掘具有优良多重耐药菌(MDR)抗菌活性的次级代谢产物,为海洋新药研发提供分子多样性。方法 对海洋芽孢杆菌B-9987进行大发酵,将发酵产物进行提取分离,每一步分离过程进行MDR活性追踪。结果 B-9987粗提物对4株MDR菌(金黄色葡萄球菌CCARM 3090、沙门氏菌CCARM 8250、大肠杆菌CCARM 1009、粪肠球菌CCARM 5203)具有良好的抗菌活性。通过HPLC分离纯化及MS、NMR等波谱分析获得了化合物1(Macrolactin F)和化合物2(Bacillaene A)。化合物1对MDR菌没有抗菌活性。由于化合物2对光、温度、氧气十分不稳定,无法直接测试活性。对含有化合物2的组分进行活性测试,确定了B-9987的MDR菌抗菌活性来自于Bacillaene类化合物。结论 鉴定了B-9987中的抗MDR菌活性成分。 |
| English Summary: |
| Objective：Discovery of antibacterial secondary metabolites against multi-drug resistant bacteria (MDRB) from marine-derived Bacillus sp. B-9987. Method The culture broth of B-9987 was extracted with EtOAc, and the EtOAc extract was further separated by open column chromatography. The pure compounds were finally obtained by HPLC purification, and their structures were determined by combination of MS and NMR data analysis. The antibacterial activity test against MDRB was carried out in each step of the separation process. |
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