| 罗小卫,刘永宏.中国南海深海真菌Penicillium brocae SCSIO 05793的次级代谢产物研究[J].中国海洋药物,2017,36(3):23-28. |
| 中国南海深海真菌Penicillium brocae SCSIO 05793的次级代谢产物研究 |
| Study on the Secondary Metabolites from South China Sea Deep-water-derived Fungus Penicillium brocae SCSIO 05793 |
| 投稿时间：2017-01-14 修订日期：2017-03-10 |
| DOI： |
| 中文关键词: 中国南海 海洋真菌 次级代谢产物 Penicillium brocae |
| English Keywords:South China Sea marine fungi secondary metabolites Penicillium brocae |
| Fund Project: |
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| 摘要点击次数: 5570 |
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| 中文摘要: |
| 目的 对分离自中国南海500m深层海水来源真菌Penicillium brocae SCSIO 05793进行菌种鉴定及次级代谢产物研究。 方法 通过ITS序列分析并构建系统发育树来鉴定菌株，综合运用硅胶柱层析、Sephadex LH-20凝胶柱层析、中压反相柱层析以及半制备高效液相等色谱学方法对其大米发酵产物进行分离纯化，利用核磁共振、质谱、旋光等波、光谱学手段并结合理化性质及文献数据鉴定其化学结构。结果 从中分离到8个单体化合物：cyclo(L-Pro-L-Leu)(1)，cyclo-(L-pro-L-Ile)(2)，cyclo(L-Pro-L-Phe)(3)，cyclo(L-Pro-L-Tyr)(4)，3,4-二羟基苯甲酸甲酯 (5)， 4-羟基苯甲醛 (6)， 4-羟基苯乙酮 (7)， 对羟基苯乙酸甲酯 (8)。结论 从该菌株Penicillium brocae中分离得到8个化合物。 |
| English Summary: |
| Objective To investigate the secondary metabolites of strain Penicillium brocae SCSIO 05793 and to identify the strain, a fungus isolated from South China Sea 500 m depth water. Methods The crude extracts were purified by comprehensive chromatography methods of silica gel column, sephadex LH-20, ostade-cylsilane (ODS) and semi-preparative HPLC and the obtained compounds’ structures were elucidated by means of spectroscopic analysis, such as NMR, MS, OR etc., together with physical and chemical properties, spectroscopic analysis and comparison with the reported literatures. Results Eight compounds were obtained from the rice static fermentation products, which were identified as cyclo(L-Pro-L-Leu)(1), cyclo-(L-pro-L-Ile)(2), cyclo(L-Pro-L-Phe)(3), cyclo(L-Pro-L-Tyr)(4), 3, 4-dihydroxy-acetophen- one(5), 4-hydroxybenzal-dehyde(6), 4-hydroxyacetophenone (7), p-hydroxyphenylacetic acid methyl ester (8). Conclusion eight compounds were isolated from the strain Penicillium brocae. |
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