| 林存智,朱新红,邵明菊,等.仿刺参糖胺聚糖对肺癌患者外周血体外细胞免疫调节功能研究[J].中国海洋药物,2017,36(4):61-65. |
| 仿刺参糖胺聚糖对肺癌患者外周血体外细胞免疫调节功能研究 |
| The research of Glycosaminoglycan from Apostichopus Japonius on cellular immuno-regulation function of peripheral blood cells from lung cancer patients in vitro |
| 投稿时间：2017-02-04 修订日期：2017-04-02 |
| DOI： |
| 中文关键词: 仿刺参糖胺聚糖 肺癌 外周血细胞 细胞免疫调节 |
| English Keywords:Apostichopus Japonius, Glycosaminoglycan lung cancer Peripheral blood cells cellular immuno-regulation |
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| 中文摘要: |
| 摘要： 目的 探讨仿刺参糖胺聚糖(HGAG)对肺癌患者外周血体外细胞免疫功能影响，为临床肿瘤免疫治疗提供理论指导。方法 选取健康人群40名（健康组）和肺癌患者30例（肺癌组）抗凝外周血4mL，应用淋巴细胞分离液采用Ficoll密度梯度离心法分离PBMC，体外与不不同浓度HGAG共培养24h。流式细胞仪检测CD45RA及CD45RO分子，以及CD1a、CD83分子的表达。结果 健康组和肺癌组CD45RA和CD45RO培养前表达差异有统计学意义（p<0.05)，培养24h后差异更加明显（p<0.001）。肺癌组体外培养24h前、后CD45RA（p<0.001）和CD45RO（p<0.05）表达差异具有统计学意义，而健康组培养前、后差异也具有统计学意义（p＜0.05）。不同浓度比较，肺癌组CD45RA和CD45RO在10μg.ml-1和50μg.ml-1组表达在无差异（p>0.05），而健康组50μg.ml-1的CD45RO表达有差异性（p<0.001），CD45RA表达无差异性（p>0.05）。健康组与肺癌组在培养前、后比较CD1a表达差异无统计学意义（p>0.05），CD83表达在培养前两组有差异性（p＜0.001），与50μg?mL-1HGAG培养24h后两组表达无差异（P＞0.05）。而肺癌组CD83表达培养前、后差异有统计学意义（p＜0.001），健康组无统计学意义（P＞0.05）。结论 HGAG在一定浓度范围内可以下调CD45RA分子表达和上调CD45RO分子表达，以及促进肺癌患者DC成熟，体外调节肺癌患者的细胞免疫功能。 |
| English Summary: |
| Abstract: Objective To investigate the effect of Glycosaminoglycan from Apostichopus Japonius on cellular immuno-regulation function for Peripheral blood cells from lung cancer patients in vitro, for providing theory guide to clinical tumor immunotherapy. Methods 40 health persons (health group) and 30 lung cancer patients (lung cancer group) were selected and took anticoagulation Peripheral blood 4mL. We separated peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation using Lydroxypropylmethyl Cellulose, and to co-culture for 24 hours with different density HGAG in vitro. To detect the express of CD45RA, CD45RO molecules, CD1a and CD83 molecules by flow cytometry. Results The express of CD45RA and CD45RO have statistical significance before cultivation (p<0.05), but the statistical significance was more obviously after cultivation 24 hours (p<0.001) in the two groups. There was statistical difference in CD45RA（p<0.001）and CD45RO（p<0.05）before and after cultivation 24 hours in lung cancer group, and the same as in health group (p＜0.05). But compare with different concentration, the express of CD45RA and CD45RO had no statistical significance in 10μg.ml-1 and 50μg.ml-1(p>0.05) in lung cancer group, but the express of CD45RO had statistical significance in 50μg.ml-1 HGAG （p<0.001）, CD45RA not in 10μg.ml-1 HGAG (P>0.05) in health group. The express of CD1a had no significance in the two groups before and after cultivation with 50μg?mL-1 HGAG (p>0.05), while the express of CD83 had significance before cultivation (p＜0.001). But after 24 hours cultivation, the express of CD83 had no significance in the two group (P＞0.05). While it had significance in lung cancer group (p＜0.001), not in health group (P＞0.05). Conclusion HGAG can down regulation CD45RA and up-regulation CD45RO expression, as well as promote DC mature and improve cellular immune function for lung cancer patients in vitro in a certain concentration. |
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