| 齐佳,王广策,郑国亚,等.岩藻黄素对胰岛素抵抗HepG2细胞葡萄糖消耗的影响[J].中国海洋药物,2017,36(5):49-54. |
| 岩藻黄素对胰岛素抵抗HepG2细胞葡萄糖消耗的影响 |
| Effect of Fucoxanthin on the Glucose Consumption of Insulin-Resistant HepG2 Cells |
| 投稿时间：2017-06-07 修订日期：2017-06-21 |
| DOI： |
| 中文关键词: 岩藻黄素 胰岛素抵抗HepG2细胞 葡萄糖消耗 |
| English Keywords:fucoxanthin insulin-resistant HepG2 cell glucose consumption |
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| 中文摘要: |
| 摘要：目的 建立胰岛素抵抗细胞模型，探讨岩藻黄素体外改善胰岛素抵抗及降血糖活性。方法 采用高浓度胰岛素诱导HepG2细胞，筛选最佳诱导条件建立IR-HepG2细胞模型。用不同浓度岩藻黄素处理模型细胞，用葡萄糖氧化酶法（GOD-POD) 检测岩藻黄素对IR-HepG2细胞葡萄糖消耗量的影响。结果 10-6 mol/L胰岛素处理细胞24 h为建立IR-HepG2细胞模型的最佳条件。5~20 μmol/L的岩藻黄素均可促进IR-HepG2细胞对葡萄糖的利用，10、12.5和15 μmol/L组的糖消耗量显著高于模型组(P<0.01)。结论 岩藻黄素可改善体外IR-HepG2细胞的胰岛素抵抗，促进IR-HepG2细胞的葡萄糖消耗，具有改善胰岛素抵抗和降血糖活性。 |
| English Summary: |
| Abstract: Objectives To establish insulin-resistant HepG2 cell model and study the effect of fucoxanthin for improving the insulin resistance and the hypoglycemic activity in vitro. Methods High concentrations of insulin were used to induce the insulin resistance in HepG2 Cells. The best concentration and induction time of insulin were selected to establish the insulin-resistant HepG2 Cell model (IR-HepG2). IR-HepG2 cells were treated with different concentrations of fucoxanthin. The glucose consumption of different groups was tested by glucose oxidase-peroxidase method (GOD-POD). Results The best concentration and treatment time of insulin to induce insulin resistance in HepG2 cells were 10-6 mol/L and 24 h. Results of the glucose consumption test showed that fucoxanthin in the concentration range of 5~20 μmol/L could stimulate the glucose utilization of the IR-HepG2 cells. Glucose consumption in the groups of 10, 12.5 and 15 μmol/L of fucoxanthin were all significantly higher than that of the IR-HepG2 model group (P<0.01). Conclusion Fucoxanthin could improve the insulin resistance and promote the glucose consumption of IR-HepG2 cells in vitro. Fucoxanthin has hypoglycemic activity. |
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