| 李松威,姚励功,梁林富,等.西瑁岛软珊瑚Sinularia sp. 的化学成分和生物活性研究[J].中国海洋药物,2018,37(4):1-6. |
| 西瑁岛软珊瑚Sinularia sp. 的化学成分和生物活性研究 |
| Study on chemical constituents and bioactivities of soft coral Sinularia sp. from Ximao Island |
| 投稿时间：2018-03-09 修订日期：2018-05-16 |
| DOI： |
| 中文关键词: 软珊瑚，甾体，细胞毒 |
| English Keywords:soft coral, steroids, cytotoxicity |
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| 中文摘要: |
| 摘要：目的 对采自中国南海西瑁岛的软珊瑚Sinularia sp.的化学成分和生物活性进行研究。方法 综合利用薄层色谱、硅胶柱色谱、凝胶柱层析、半制备HPLC等分离手段，结合NMR和MS等波谱数据分析并对比文献数据，对化合物进行分离鉴定；选用A549和HL-60细胞株，采用CCK-8筛选方法，对化合物进行体外细胞毒活性测试。结果 从软珊瑚Sinularia sp.中共分离鉴定了10个甾体化合物，包括8个孕甾烷及其苷类化合物：3β-羟基-5,20-二烯孕甾烷乙酸酯（1），20-烯-3-酮孕甾烷（2），1,20-二烯-3-酮孕甾烷（3），1,4,20-三烯-3-酮孕甾烷（4），3β-羟基-20-烯孕甾烷（5），3β-羟基-5,20-二烯孕甾烷（6），1,4-二烯-3-酮-20α-羟基孕甾烷乙酸酯（7），3β-羟基-5,20-二烯孕甾烷吡喃岩藻糖苷（8）；1个麦角甾烷类化合物：(22E,24R)-3β,5α,6β-三羟基-7,22-二烯麦角甾烷（9）和1个胆甾烷类化合物：(20S)-1,4-二烯-3,16-二酮-20β-羟基胆甾烷（10）。结论 化合物1～10对A549细胞株均未表现明显的细胞毒活性，但化合物4和7对HL-60细胞株具有中等的细胞毒活性，其IC50值分别为1.61 和 3.26 μmol/L。 |
| English Summary: |
| Abstract: Objective To study the chemical constituents and bioactivities of soft coral sinularia sp. collected from Ximao Island in South China Sea. Methods The isolation and purification of these compounds were performed by TLC, silica gel, Sephadex LH-20 column chromatography and semi-preparative HPLC. Their structures were characterized by analysis of NMR and MS data and comparison with the data reported in literature. The cytotoxicity of these compounds against A549 and HL-60 cell lines has been tested by using Cell Counting Kit-8 (CCK-8) method. Results Ten known steroids were isolated from the soft coral sinularia sp. and their structures were identified as pregna-5,20-dien-3β-O-acetate (1), pregna-20-en-3-one (2), pregna-1,20-dien-3-one (3), pregna-1,4,20-trien-3-one (4), pregna-20-en-3β-ol (5), pregna-5,20-dien-3β-ol (6), pregna-1,4-dien-3-one-20α-O-acetate (7), α-galactopyranoside-3β-pregna-5,20-dien-3-yl-6-deoxy (8), (22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol (9) and (20S)-20-hydroxy cholost-1,4-dien-3,16-dione (10). Conclusion Though compounds 1～10 didn"t exhibit potent cytotoxicity against A549 cell lines, compounds 4 and 7 showed moderate cytotoxicity against HL-60 cell lines with IC50 values of 1.61 and 3.26 μmol/L, respectively. |
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