| 仲伟茂,曾奇,向瑶,等.南海深海沉积环境来源真菌Aspergillus sp. SCSIO F063的次生代谢产物研究[J].中国海洋药物,2018,37(5):29-35. |
| 南海深海沉积环境来源真菌Aspergillus sp. SCSIO F063的次生代谢产物研究 |
| Study on the Secondary Metabolites from South China Sea Deep-sea Sediment Derived Fungus Aspergillus sp. SCSIO F063 |
| 投稿时间：2018-04-11 修订日期：2018-05-08 |
| DOI： |
| 中文关键词: 深海真菌 OSMAC 次生代谢产物 抗氧化活性 |
| English Keywords:deep-sea derived fungus OSMAC secondary metabolites antioxidative activities |
| Fund Project: |
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| 中文摘要: |
| 目的 采用单株菌多次级代谢产物(OSMAC)策略对一株南海深海沉积环境来源真菌的次生代谢产物进行分离、鉴定及活性研究。方法 通过改变培养基组成并筛选合适的发酵条件,采用硅胶柱层析、反相ODS柱层析、半制备高效液相等色谱学方法对真菌Aspergillus sp. SCSIO F063的发酵产物进行化学分离,利用NMR, MS等波谱学技术并结合文献进行化合物的结构鉴定,并对化合物进行初步的抗氧化活性测试。结果 从菌株SCSIO F063中新增分离鉴定5个单体化合物： 6-O-methyl-averythrin(1), (2,4-dichlorophenyl)-2,4-dichlorobenzoate(2),dibutyl phthalate(3),folipastatin(4),di-(2-ethylhexyl)-phthalate(5)。结论 改变培养基组成可以刺激该菌株产生不同类型的化合物,化合物1-5为首次从真菌SCSIO F063中分离得到。 |
| English Summary: |
| Objective Using one strain many compounds strategy to isolate and identify the secondary metabolites of strain Aspergillus sp. SCSIO F063, a deep-sea sediment derived fungus from South China Sea. Methods By altering the cultivation parameters and screening the fermentation conditions, the extracted products were purified by silica gel column, reverse phase ODS column, and semi-preparative HPLC. The isolated compounds were identified by spectroscopic analysis (NMR and MS) as well as comparison with literature. They were screened for antioxidative potency. Results Five compounds were obtained from the ethyl acetate extracts of strain SCSIO F063. Their structures were identified as 6-O-methyl-averythrin(1),(2,4-dichlorophenyl)-2,4-dichlorobenzoate(2),dibutyl phthalate(3),folipastatin(4),di-(2-ethylhexyl)-phthalate(5)。Conclusion Using different medium in fermentation of this fungus could result different kinds of secondary metabolites. Compounds 1-5 were isolated from SCSIO F063 for the first time. |
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