| 陈祥艳,韩文伟,赵峡,等.表阿霉素卡拉胶寡糖-金纳米粒的制备和质量控制[J].中国海洋药物,2018,37(5):67-73. |
| 表阿霉素卡拉胶寡糖-金纳米粒的制备和质量控制 |
| Preparation and quality control of epirubicin carrageenan oligosaccharide Au nanoparticles |
| 投稿时间：2018-04-27 修订日期：2018-05-29 |
| DOI： |
| 中文关键词: 表阿霉素 金纳米粒 卡拉胶寡糖 高效液相色谱法 含量测定 |
| English Keywords:epirubicin gold nanoparticles carrageenan oligosaccharide HPLC content determination |
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| 中文摘要: |
| 目的 建立盐酸表阿霉素-卡拉胶寡糖-金纳米（EPI-CAO-AuNPs）中盐酸表阿霉素（EPI）的含量测定方法，并测定其包封率。方法 采用ZORBAX-Extend-C18色谱柱（4.6 ×150 mm，5 μm），以乙腈和水为流动相，对检测波长、流动相比例及pH、流速和柱温等因素进行优化。结果 优化后最适色谱条件为：流动相为乙腈/水（30/70，V/V），含0.1 %的三氟乙酸（TFA），柱温25 ℃，流速1.0 mL/min，检测波长233 nm。经测定，EPI-CAO-AuNPs中EPI的载药量为12.5 %，包封率为94.3 %。结论 该测定方法操作简单，快速灵敏，重复性好，可在15 min内完成测定，适用于EPI-CAO-AuNPs材料中EPI的含量检测。 |
| English Summary: |
| To establish a HPLC method for the content determination of epirubicin (EPI) in EPI-CAO-AuNPs. METHOD: ZORBAX-Extend-C18 column (4.6 × 150 mm, 5 μm) was used for the determination of EPI with acetonitrile and water as the mobile phase. The chromatographic conditions including the detection wavelength, the ratio and pH of mobile phase, flow rate, and column temperature were optimized. RESULTS: The optimized chromatographic conditions were as follows: the volume ratio of acetonitrile/water was 30/70 with 0.1% trifluoroacetic acid (TFA), the column temperature was 25 °C, the flow rate was 1.0 mL/min, the running time was 15 min, and the detection wavelength was 233 nm. The drug loading of EPI in EPI-CAO-AuNPs was 12.5 %, and the encapsulation efficiency of that was 94.3 %. CONCLUSIONS: The established and optimized method was accurate, reliable and simple, which can be completed within 15 min. It is suitable for the content determination of EPI in EPI-CAO-AuNPs. |
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