| 季宇彬,关鑫,尹姣定,等.一种快速筛选抗菌活性海洋真菌的方法[J].中国海洋药物,2019,38(2):35-38. |
| 一种快速筛选抗菌活性海洋真菌的方法 |
| A method for rapid screening of antibacterial marine fungal strains |
| 投稿时间：2018-11-15 修订日期：2019-02-28 |
| DOI： |
| 中文关键词: 海洋真菌 抗菌活性 筛选方法 |
| English Keywords:marine fungi antibacterial activity screening method |
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| 中文摘要: |
| 摘要：目的 建立一种快速筛选抗菌活性真菌菌株的方法 方法 设计并改良了一种双层琼脂板快速筛选方法 结果 利用改良双层琼脂板筛选法，从一批真菌中快速筛选获得一株具有显著抗菌活性的菌株WZXY-33-10，并通过现代色谱学及波谱学方法从其发酵物中分离鉴定了2个抗菌活性化合物equisetin（1）和epi-equisetin（2）。结论 改良双层琼脂板筛选法可以作为一种快速发现抗菌活性海洋真菌的方法。 |
| English Summary: |
| Abstract: Objective To establish a rapid screening method for fungal strains with antibacterial activity. Methods A double-layer plate method was designed for the rapid screening of antibacterial fungal strains. Results One antibacterial strain WZXY-33-10 was selected out from dozens of fungal strains by double-layer plate screening assay for the purification of antibiotics. Additionally, two antibacterial compounds equisetin (1) and epi-equisetin (2) were isolated from the fermentation extract of WZXY-33-10 by modern chromatography methods. Conclusion The double-layer plate screening can be a rapid method for screening of antibacterial marine fungal strains. |
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